Shake Flask Solubility Service

The solubility of a drug compound plays a significant role in absorption and is an important factor to consider during development. Quantitative measurement of compound solubility is an ideal early test to perform when screening for potential drug candidates.

BioAssay Systems (BAS) offers two methods for quantifying the solubility of drug compounds: filter plate solubility testing and shake flask solubility testing. This information page details the Shake Flask Method.

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In 2 mL tubes, an excess of the test compound is weighed out and mixed with a solvent of customer's choice. The tubes are shaken for 18 hours (or desired duration) at ambient temperature (or desired temperature). The solution is vacuum filtered to remove precipitates. Absorbance spectrum is run on the filtrate together with the test compound standards. Concentration of the filtrate is calculated using the slope of the standards.

Shake Flask figure1           Shake Flask figure2
Figure 1: Drug compound solubility determined using the shake flask method. Testing was repeated three times on separate days. Experimentally determined solubility of drug compounds using the shake flask method were compared to literature values.1,2

1. Wishart DS, Feunang YD, Marcu A, Guo AC, Liang K, et al., HMDB 4.0 β€” The Human Metabolome Database for 2018. Nucleic Acids Res. 2018. Jan 4;46(D1):D608-17. 29140435
2. National Center for Biotechnology Information. PubChem Compound Database; CID=5359476, (accessed May 22, 2018).

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Edward T. Wei, Chief Scientific Officer, Orinda Pharma, Inc.
β€œIt is a pleasure working together with BioAssay Systems. I brought samples to their Hayward location and received the results in less than 24 hr. The discussion with Robert Z was courteous, open, and with a depth of knowledge. Robert has a PhD from Stanford. Also, Frank Huang, the CEO, had sufficient scientific curiosity to try out our cleanser and provide feedback! This is beyond the call of customer relations. BioAssay Systems is a company with a lot of expertise and a friendly, responsive approach to problem solving. I will enthusiastically continue to work with them to carry out our research.”

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