PAMPA Permeability Service

PAMPA Permeability Service

Summary
Due to the membrane barriers most drugs must pass through to reach their target, the permeability of a drug compound is an important factor to consider during development. Quantitative measurement of compound permeability is an ideal early test to perform when screening for potential drug candidates.

One of the Permeability tests BioAssay Systems offers is the Parallel Artificial Membrane Permeability Assay (PAMPA). This test measures passive diffusion of compounds and is ideal for early stage drug development because it is fast and low cost relative to other cell-based permeability assays.

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Method
A membrane mimicking solution and full permeability control solution are loaded on to separate well membranes of a PAMPA donor plate. The donor plate is then loaded with the desired concentration of test compound in a buffer of customer's choice. The receiver plate is loaded with either the same buffer or another of the customer’s choice. The donor plate is gently placed into the receiver plate and incubated for a set duration at ambient temperature (or desired temperature).

After the incubation, the solutions in the receiver plate are collected and analyzed by UV-spectroscopy alongside test compound standards in DMSO. The test compound permeability is calculated by comparing the membrane mimicking system to that of the full permeability control

PAMPA figure1     PAMPA figure2
Figure 1 & 2: Drug compound permeability determined using the PAMPA method. Testing was repeated three times on separate days.

PAMPA figure3
Figure 3: Diagram of PAMPA method.


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Edward T. Wei, Chief Scientific Officer, Orinda Pharma, Inc.
“It is a pleasure working together with BioAssay Systems. I brought samples to their Hayward location and received the results in less than 24 hr. The discussion with Robert Z was courteous, open, and with a depth of knowledge. Robert has a PhD from Stanford. Also, Frank Huang, the CEO, had sufficient scientific curiosity to try out our cleanser and provide feedback! This is beyond the call of customer relations. BioAssay Systems is a company with a lot of expertise and a friendly, responsive approach to problem solving. I will enthusiastically continue to work with them to carry out our research.”

Paul Abbyad, Ph.D., Assistant Professor, Santa Clara University
"I would like to show my appreciation to the Bioassay Systems service team. We are developing an assay to measure the change in lactate concentration of single cell samples using fluorescent microscopy. The BioAssay Systems team was quite helpful in assisting us to optimize their fluorescent L-Lactate assay kit for our unique experimental set-up. The BioAssay Systems team is always friendly and knowledgeable. We know that we can rely on them for fast and professional service."