Filter Plate Solubility Service

Summary
The solubility of a drug compound plays a significant role in absorption and is an important factor to consider during development. Quantitative measurement of compound solubility is an ideal early test to perform when screening for potential drug candidates.

BioAssay Systems (BAS) offers two methods for quantifying the solubility of drug compounds: filter plate solubility testing and shake flask solubility testing. This information page details the Filter Plate Method.

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Method
In MultiScreen filter plate, the compound is mixed with a solvent of customer's choice. The plate is gently shaken for 1.5 hours at ambient temperature (or desired temperature). The solution is vacuum filtered. Absorbance spectrum is run on the filtrate together with the test compound standards. Concentration of the filtrate is calculated using the slope of the standards.

filter plate figure1           filter plate figure2
Figure 1: Drug compound solubility determined using the filter plate method. Testing was repeated three times on separate days. Experimentally determined solubility of drug compounds using the filter plate method were compared to literature values.1

Literature
1. Pan, L. et al. (2000). Comparison of Chromatographic and Spectroscopic Methods Used to Rank Compounds for Aqueous Solubility. J. Pharm. Sci. 90:4, 521-529.


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Edward T. Wei, Chief Scientific Officer, Orinda Pharma, Inc.
“It is a pleasure working together with BioAssay Systems. I brought samples to their Hayward location and received the results in less than 24 hr. The discussion with Robert Z was courteous, open, and with a depth of knowledge. Robert has a PhD from Stanford. Also, Frank Huang, the CEO, had sufficient scientific curiosity to try out our cleanser and provide feedback! This is beyond the call of customer relations. BioAssay Systems is a company with a lot of expertise and a friendly, responsive approach to problem solving. I will enthusiastically continue to work with them to carry out our research.”

Paul Abbyad, Ph.D., Assistant Professor, Santa Clara University
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