QuantiQuik™ Histamine Quick Test Strips
Protocol SDS Available on Amazon Right now on eBay   Printed Protocols and SDSs are not provided with kits.

Application
Direct determination of Histamine concentrations in fish and red wine samples.

Key Features
Fast and sensitive. Use of 500 mg of fish or 250 µL of sample. Semi-quantitative measurement between 0-200 ppm (undiluted) Histamine.

Convenient. No expensive lab equipment needed.

Sample treatment and assay can be performed in under 15 minutes.

Method
Visual

Samples
Seafood, wine, milk, etc

Species
All

Procedure
Approximately 15 min

Size
10 tests

Shelf Life
6 months

More Details
HISTAMINE(C5H9N3) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant mostly found in Scombridae family fishes, such as tuna and mackerel, as well as other seafood products when they are improperly handled or stored. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning known as scombroid poisoning.

BioAssay Systems QuantiQuik™ Histamine Quick Test Strips are based on histamine dehydrogenase catalyzed oxidation of histamine in which the formed electron mediator reduces a formazan (MTT) reagent. The intensity of product color is directly proportional to histamine concentration in the sample.

1. What samples have you tested?



Fish samples: Ahi tuna, Mahi Mahi, Albacore, Mackerel, Anchovy, Salmon, Tilapia. Red wine, white wine, and milk.

2. How sensitive are the Histamine strips?



The lower detection limit of the strips for seafood, red wine, and milk samples is 20 ppm.

3. My sample turns the strip very dark purple, how can I determine the Histamine concentration?



Very dark purple indicates that the diluted sample concentration is greater than 200 ppm. To obtain a more accurate concentration, the sample should be diluted and retested. Multiply the concentration of the corresponding color by the dilution factor to determine the histamine concentration.

4. I don’t have access to a homogenizer. How can I effectively homogenize my fish samples?



Fish samples can be mashed with a tooth pick and then vigorously shaken for 2 minutes in the extraction buffer.

5. How can I measure levels of histamine in milk samples?



Milk samples require a 2× Dilution. Carefully add 200 µL of Extraction Buffer to a Sample tube. Then, add 200 µL of milk. Replace the cap, securely close the vial, and invert the vial a couple of times to mix the diluted sample. Proceed with step 1 of the test procedure on the product protocol. Dilution Factor=2

6. Can I measure histamine in white wine samples?



Histamine can be measured in white wine samples, but please note that only levels greater than 20 ppm can be detected and white wine rarely has levels of histamine this high. White wine samples should be diluted 2× in Extraction buffer. See question 5 for instructions.

7. Can I store unused reagents for future use?



Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, preferably cool location protected from light. For long term storage (>30 days), we recommend keeping the Extraction Buffer at 4°C or below.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
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Please inquire or request assay service or call 1-510-782-9988 x 2.
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QuantiQuik™ Histamine Quick Test Strips
Catalog No: QQHIST10
Price: $49    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: Free Shipping
Shipment: USPS
Delivery: 2-5 days (Continental US only) Storage: RT

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