QuantiChrom™ Zinc Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of zinc ion Zn2+ and evaluation of drug effects on zinc metabolism.

Key Features
Sensitive and accurate. Uses 50 μL samples. Linear detection range 0.12 μM (0.78 μg/dL) to 10 μM (65 μg/dL) zinc in 96-well assay format.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible.

Low interference in biological samples. No pretreatments are needed.

Method
OD425nm

Samples
Serum, plasma, urine, saliva, food, beverage and environment

Species
All

Procedure
30 min

Size
250 tests

Detection Limit
0.78 μg/dL (0.12 μM)

Shelf Life
6 months

More Details
Zinc is an essential trace element and plays many key roles in metabolism. It is required for the activity of more than 300 enzymes, the structure of many proteins, and control of genetic expression. Zinc status affects basic processes of cell division, growth, differentiation, development, performance and aging through its requirement for synthesis and repair of DNA, RNA and protein. The common causes of zinc deficiency are low dietary intakes and low bioavailability. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Zinc deficiency has now been recognized to be associated with many diseases such as malabsorption syndrome, chronic liver disease, chronic renal disease, sickle cell disease, diabetes, malignancy, and other chronic illnesses. Simple, direct and automation-ready procedures for measuring zinc concentration in biological samples are highly desirable in Research and Drug Discovery. BioAssay Systems zinc assay kit is designed to measure zinc directly in biological samples without any pretreatment. The present method utilizes a chromogen that forms a colored complex specifically with zinc. The intensity of the color, measured at 425 nm, is directly proportional to the zinc concentration in the sample.

1. Our plate reader does not have a filter for 425 nm, can I use 405nm or 450 nm instead?



No, the zinc assay must be measured between 419 nm and 426 nm. Outside of this narrow range the assay will not work.

2. How many standards (to generate a graph) should I include in the assay?



Because this assay is not linear from 0.12 to 10 µM, please follow the protocol and run standard curve for each assay.

3. Do I need to dilute urine samples for this assay?



No, the zinc concentration in urine is sufficiently low.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Bergeron, N., & Guay, F. (2019). Impact of zinc and arginine on antioxidant status of weanling piglets raised under commercial conditions. Animal Nutrition. Assay: Zinc in pig plasma.

Boudreault, F., Pinilla-Vera, M., Englert, J. A., Kho, A. T., Isabelle, C., Arciniegas, A. J. & Choi, K. M. (2017). Zinc deficiency primes the lung for ventilator-induced injury. JCI insight, 2(11). Assay: Zinc in mice blood.

Dambal, S., Baumann, B., McCray, T., Williams, L., Richards, Z., Deaton, R. & Nonn, L. (2017). The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts. Scientific reports, 7(1), 7704. Assay: Zinc in rat tumor cells.

White, J. R., Gong, H., Pope, B., Schlievert, P., & McElroy, S. J. (2017). Paneth-cell-disruption-induced necrotizing enterocolitis in mice requires live bacteria and occurs independently of TLR4 signaling. Disease models & mechanisms, 10(6), 727-736. Assay: Zinc in mice blood.

Xu, J et al (2014). Nanosized zinc oxide particles do not promote DHPN-induced lung carcinogenesis but cause reversible epithelial hyperplasia of terminal bronchioles. Archives of toxicology 88(1): 65-75. Assay: Zinc in rat lung tissue.

Kelly, E., Mathew, J., Kohler, J. E., Blass, A. L., & Soybel, D. I. (2012). Hemorrhagic Shock and Surgical Stress Alter Distribution of Labile Zinc within High and Low Molecular Weight Plasma Fractions. Shock (Augusta, Ga.) 38(3): 314. Assay: Zinc in rat plasma.

Leung, KW, et al. (2012). ZIP2 and ZIP4 Mediate Age-Related Zinc Fluxes Across the Retinal Pigment Epithelium. J Mol Neurosci. 46(1): 122-137. Assay: Zinc in human retinal pigment epithelial cells.

Gunasekara, P, et al. (2011). Effects of zinc and multimineral vitamin supplementation on glycemic and lipid control in adult diabetes. Diabetes Metab Syndr Obes 4:53-60. Assay: Zinc in human serum, urine.

Kelly, E, et al. (2011). Redistribution of labile plasma zinc during mild surgical stress in the rat. Transl Res 157(3):139-49. Assay: Zinc in rat plasma.

Leung, KW et al (2011). ZIP2 and ZIP4 Mediate Age-Related Zinc Fluxes Across the Retinal Pigment Epithelium. J Mol Neurosci . Assay: Zinc in human Retinal pigment epithelial cells.

Mihelich, BL et al (2011). miR-183-96-182 cluster is overexpressed in prostate tissue and regulates zinc homeostasis in prostate cells. Journal of Biological Chemistry 286(52): 44503-44511. Assay: Zinc in human prostate cells.

Morgan, C. I., Ledford, J. R., Zhou, P., & Page, K. (2011). Zinc supplementation alters airway inflammation and airway hyperresponsiveness to a common allergen. J. Inflamm. (Lond.) 8: 36. Assay: Zinc in mouse serum.

Padiglia, A., et al. (2010). Sensitivity to 6-n-propylthiouracil is associated with gustin (carbonic anhydrase VI) gene polymorphism, salivary zinc, and body mass index in humans. Am J Clin Nutr 92(3):539-45. Assay: Zinc in human saliva.

Knoell, DL, et al. (2009). Zinc deficiency increases organ damage and mortality in a murine model of polymicrobial sepsis. Crit Care Med 37(4):1380-8. Assay: Zinc in mouse serum.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
QuantiChrom™ Zinc Assay Kit
Catalog No: DIZN-250
Price: $409    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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