QuantiChrom™ Uric Acid Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism.

Key Features
Sensitive and accurate. Use 5 μL samples. Linear detection range 0.22 mg/dL (13 μM) to 30 mg/dL (1785 μM) uric acid in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.

Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.

Method
OD590nm

Samples
Serum, plasma, urine and other biological samples

Species
All

Procedure
30 min

Size
250 tests

Detection Limit
0.2 mg/dL (13 μM)

Shelf Life
12 months

More Details
Uric acid is the waste product produced from the degradation of purines. In healthy human, uric acid is filtered and removed from the blood by the kidneys and excreted into urine. Because a number of kidney diseases are known to affect uric acid levels, uric acid determination is thus important and useful in diagnosing and evaluating kidney diseases. For example, when uric acid is present in the blood at abnormally high levels, it tends to crystallize in body joints, resulting in gout, a very painful inflammatory condition. Increased levels of uric acid are also known to be associated with uremia, leukemia and pneumonia. Simple, direct and automation-ready procedures for measuring uric acid concentration in blood are becoming popular in Research and Drug Discovery. BioAssay Systems’ uric acid assay kit is designed to measure uric acid directly in serum without any pretreatment. The improved method utilizes 2,4,6-tripyridyl-s-triazine that forms a blue colored complex specifically with iron in the presence of uric acid. The intensity of the color, measured at 590nm, is directly proportional to the uric acid concentration in the serum. The optimized formulation substantially reduces interference by substances in the raw samples.

1. What is the principle of the assay?



The assay is based on the quantitative reduction of Fe(III) to Fe(II) by uric acid . Fe(II) reacts with 2,4,6 - tripyridyl-s-triazine to form a colored complex which is measured colorimetrically. The color intensity is directly proportionate to the uric acid concentration.

2. How specific is the assay?



The assay is not specific for uric acid, but will also detect other reducing substances (antioxidants), such as ascorbic acid. In human serum and urine samples uric acid is the predominant antioxidant. Most mammals, except for great apes, produce the enzyme urate oxidase, and serum uric acid levels are much lower (about 1/5th of human serum levels). In those serum samples the assay may overestimate the actual uric acid concentration.

3. Can I use this kit to determine uric acid levels in cow manure?



Yes. You can extract uric acid with 7 mM glycine buffer, pH 9.5 containing 5% glycerol. Simply mix preweighed manure and known amount of buffer together, homogenize in a tissue homogenizer or shake to dissolve all solid materials. Filtrate or centrifuge to obtain clear extract.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Kono, H et al (2010). Uric acid promotes an acute inflammatory response to sterile cell death in mice. J Clin Invest 120(6):1939-49. Assay: Uric Acid in Mouse Peritoneal lavage fluid (Pubmed).

2. Thangaraju, M et al (2006). c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney. J Biol Chem 281(37):26769-73. Assay: Uric Acid in Mouse Serum, Urine (Pubmed).

3. Brosnahan, AJ et al (2009). Cytolysins augment superantigen penetration of stratified mucosa. J Immunol 182(4):2364-73. Assay: Uric Acid in Human Vaginal epithelial cells (Pubmed).

4. Yu, MA et al (2010). Oxidative stress with an activation of the renin-angiotensin system in human vascular endothelial cells as a novel mechanism of uric acid-induced endothelial dysfunction. J Hypertens 28(6):1234-42. Assay: Uric Acid in Human endothelial cell (Pubmed).

5. Xu, X et al (2011). Major histocompatibility complex class I-related chain A/B (MICA/B) expression in tumor tissue and serum of pancreatic cancer: role of uric acid accumulation in gemcitabine-induced MICA/B expression. BMC Cancer 11(1):194. Assay: Uric Acid in Human PANC-1 pancreatic cancer cell lysate (Pubmed).

6. Kaya, H et al (2008). The protective effect of N-acetylcysteine against cyclosporine A-induced hepatotoxicity in rats. J Appl Toxicol 28(1):15-20. Assay: Uric Acid in Rat Serum (Pubmed).

7. Kamel, AH (2007). Conventional and planar chip sensors for potentiometric assay of uric acid in biological fluids using flow injection analysis. J Pharm Biomed Anal 45(2):341-8. Assay: Uric Acid in Human Urine (Pubmed).

8. Whidden, MA et al (2009). Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction. J Appl Physiol 106(2):385-94. Assay: Uric Acid in Rat Diaphragm (Pubmed).

9. Moon, HG et al (2010). Conversion of Th17-type into Th2-type inflammation by acetyl salicylic acid via the adenosine and uric acid pathway in the lung. Allergy 65(9):1093-103. Assay: Uric Acid in Mouse lung tissue (Pubmed).

10. Belarbi, M et al (2011). Oleaster Oil Positively Modulates Plasma Lipids in Humans. J Agric Food Chem . Assay: Uric Acid in Human Plasma (Pubmed).

11. Viel, EC et al (2008). Xanthine oxidase and mitochondria contribute to vascular superoxide anion generation in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol 295(1):H281-8. Assay: Uric Acid in Rat Plasma (Pubmed).

12. DiSilvestro RA, DiSilvestro DJ (2009). Pomegranate extract mouth rinsing effects on saliva measures relevant to gingivitis risk. Phytother Res 23(8):1123-7. Assay: Uric Acid in Human Saliva (Pubmed).

13. Karaouzene, N et al (2011). Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: A comparison of older with young men. Nutr Metab Cardiovasc Dis . Assay: Uric Acid in Human Plasma (Pubmed).

14. Smith, CL et al (2011). Naturally high plasma glucose levels in mourning doves (Zenaida macroura) do not lead to high levels of reactive oxygen species in the vasculature. Zoology (Jena) 114(3):171-6. Assay: Uric Acid in Bird Plasma (mourning dove) (Pubmed).

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QuantiChrom™ Uric Acid Assay Kit
Catalog No: DIUA-250
Price: $329    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C



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