QuantiChrom™ Urea Assay Kit

QuantiChrom™ Urea Assay Kit
Catalog No: DIUR-100
Price: $146    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: 4, -20°C
Urea Assay Kit
Protocol SDS

Application

  • For quantitative determination of urea and evaluation of drug effects on urea metabolism.

Key Features

  • Sensitive and accurate. Use 5 μL samples. Linear detection range 0.08 mg/dL (13 μM) to 100 mg/dL (17 mM) urea in 96-well plate assay.
  • Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
  • Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
  • Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.

Method

  • OD520nm (Chemical)

Samples

  • Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.08 mg/dL (13 μM)

Shelf Life

  • 12 months

More Details

  • Urea is primarily produced in the liver and secreted by the kidneys. Urea is the major end product of protein catabolism in animals. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases, e.g., congestive heart failure, liver diseases and diabetes. Decreased levels indicate acute hepatic insufficiency or may result from over-vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea concentration or blood urea nitrogen BUN in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems urea assay kit is designed to measure urea directly in biological samples without any pretreatment. The improved Jung method utilizes a chromogenic reagent that forms a colored complex specifically with urea. The intensity of the color, measured at 520nm, is directly proportional to the urea concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

What is the principle of the urea assay?

The reaction proceeds with two steps and is based on a method developed by Jung et al. The first step is the condensation reaction of ortho-phthaldialdedyde with urea. The second step involves the rapid reaction with primaquine diphosphate that undergoes rearrangement to yield an intensely colored product.

I wonder if your urea assay kit could measure urea concentrations in dairy cow urine? Total nitrogen runs 240 grams per dL. Urea runs 50-70% of this amount, depending on ration components.

Yes, you can measure urea in cow urine with our urea assay. We would suggest that you dilute the urine 10-fold in water prior to the assay. This will give a final concentration of 12 to 17 mg/dL and is within the linear detection range of the assay (0 to 50 mg/dL).

Is it normal that plasma samples form precipitates after the addition of working reagent?

Some plasma samples will form precipitates after the addition of the working reagent. If this is the case, we recommend to perform the reaction in a microtube centrifuge at maximum speed for five minutes and use the clear supernatant for the measurement.

I was curious if ammonia/ammonium interferes with your urea assay (DIUR-500)? I am looking for an assay to measure in vitro urea conversion from ammonium chloride within culturing media by hepatocytes, and am wondering if this assay should be applicable for this goal.

Ammonium does not interfere with the assay. Unlike the other urea assays which measure ammonium after enzymatic conversion of urea to ammonium, this assay measures urea directly. Thus you can use our urea assay for your experiment.

Does peroxide interfere with the assay?

Yes. High concentrations of hydrogen peroxide (1%) completely block color development. We have not tested lower concentrations.

Does ethanol interfere with the assay?

No. Up to 25% ethanol in the sample does not interfere with the reaction.

I am planning to measure urea from culture supernatant. My medium contains phenol red, so I am wondering how to correct for the background by phenol red?

You should measure the OD at 430 nm, because the background by phenol red will be much lower than at 520 nm. Generally, I would recommend to run the standard in phenol red containing medium and use medium as blank instead of water.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Bhogal, R.H., et al. (2011). Isolation of primary human hepatocytes from normal and diseased liver tissue: a one hundred liver experience. PLoS One 6(3):e18222. Assay: Urea in human primary hepatocytes.

Brooks, E.J., et al. (2011). The physiological response of the Caribbean reef shark (Carcharhinus perezi) to longline capture. Comp Biochem Physiol A Mol Integr Physiol 162(2):94-100. Assay: Urea in shark plasma.

Chavez Valdez, R., et al. (2011). Correlation between serum caffeine levels and changes in cytokine profile in a cohort of preterm infants. J Pediatr 158(1):57-64, 64 e1. Assay: Urea in human tracheal aspirates.

Drusano, G.L., et al. (2011). Meropenem penetration into epithelial lining fluid in mice and humans and delineation of exposure targets. Antimicrob Agents Chemother 55(7):3406-12. Assay: Urea in mouse epithelial lining fluid and plasma.

Gatti, S., et al. (2011). Microvesicles derived from human adult mesenchymal stem cells protect against ischaemia-reperfusion-induced acute and chronic kidney injury. Nephrol Dial Transplant 26(5):1474-83. Assay: Urea in rat serum.

Giguere, S., et al. (2011). Disposition of gamithromycin in plasma, pulmonary epithelial lining fluid, bronchoalveolar cells, and lung tissue in cattle. Am J Vet Res 72(3):326-30. Assay: Urea in cattle bronchoalveolar lavage fluid.

Pauwelyn, K., et al. (2011). Culture of mouse Embryonic Stem Cells with Serum but without Exogenous Growth Factors Is Sufficient to Generate Functional Hepatocyte-Like Cells. PLoS One 6(8):e23096. Assay: Urea in mouse cells.

Radunz, A.E., et al. (2011). Winter-feeding systems for gestating sheep I. Effects on pre- and postpartum ewe performance and lamb progeny preweaning performance. J Anim Sci 89(2):467-77. Assay: Urea in sheep plasma.

Sekiya S, Suzuki A (2011). Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475(7356):390-3. Assay: Urea in mouse hepatocyte cultures.

Wang, Z., et al. (2011). Actinonin, a meprin A inhibitor, protects the renal microcirculation during sepsis. Shock 35(2):141-7. Assay: Urea in mouse serum.

Wynn, T.A., et al. (2011). Quantitative assessment of macrophage functions in repair and fibrosis. Curr Protoc Immunol Chapter 14:Unit14 22. Assay: Urea in various species macrophages.

Benjamin, J.T., et al. (2010). NF-kappaB activation limits airway branching through inhibition of Sp1-mediated fibroblast growth factor-10 expression. J Immunol 185(8):4896-903. Assay: Urea in human tracheal fluid.

Ciarimboli, G., et al. (2010). Organic cation transporter 2 mediates cisplatin-induced oto- and nephrotoxicity and is a target for protective interventions. Am J Pathol 176(3):1169-80. Assay: Urea in mouse blood.

Dennen, P., et al. (2010). Urine interleukin-6 is an early biomarker of acute kidney injury in children undergoing cardiac surgery. Crit Care 14(5):R181. Assay: Urea in mouse blood.

Dickinson, G.S., et al. (2010). Toll-like receptor 2 deficiency results in impaired antibody responses and septic shock during Borrelia hermsii infection. Infect Immun 78(11):4579-88. Assay: Urea in mouse plasma.

Hauser, P.V., et al. (2010). Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery. Am J Pathol 177(4):2011-21. Assay: Urea in mouse serum.

Irani, K., et al. (2010). Mechanical dissociation of swine liver to produce organoid units for tissue engineering and in vitro disease modeling. Artif Organs 34(1):75-8. Assay: Urea in pig liver organoids.

Miller, J.D., et al. (2010). Chorioamnionitis stimulates angiogenesis in saccular stage fetal lungs via CC chemokines. Am J Physiol Lung Cell Mol Physiol 298(5):L637-45. Assay: Urea in human tracheal aspirates.

Okura, H., et al. (2010). Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells. Tissue Eng Part C Methods 16(4):761-70. Assay: Urea in human stem cells.

Park, Y., et al. (2010). Expansion and hepatic differentiation of rat multipotent adult progenitor cells in microcarrier suspension culture. J Biotechnol 150(1):131-9. Assay: Urea in rat multipotent adult progenitor cells.

Ponda, M.P., et al. (2010). Methylamine clearance by haemodialysis is low. Nephrol Dial Transplant 25(5):1608-13. Assay: Urea in human plasma.

Ponda, M.P., et al. (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210(1):57-62. Assay: Urea in mouse serum.

Roelandt P et al (2010). Differentiation of rat multipotent adult progenitor cells to functional hepatocyte-like cells by mimicking embryonic liver development. Nat Protoc. 5(7):1324-36. Assay: Urea in human cell media.

Roelandt, P., et al. (2010). human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS One 5(8):e12101. Assay: Urea in human cells.

Sahan-Firat, S., et al. (2010). 2,3',4,5'-Tetramethoxystilbene prevents deoxycorticosterone-salt-induced hypertension: contribution of cytochrome P-450 1B1. Am J Physiol Heart Circ Physiol 299(6):H1891-901. Assay: Urea in rat urine.

Watanabe, M., et al (2010). AAVrh.10-mediated genetic delivery of bevacizumab to the pleura to provide local anti-VEGF to suppress growth of metastatic lung tumors. Gene Ther 17(8):1042-51. Assay: Urea in mouse bronchoalveolar lavage fluid.

Andonegui, G., et al. (2009). Characterization of S. pneumoniae pneumonia-induced multiple organ dysfunction syndrome: an experimental mouse model of gram-positive sepsis. Shock 31(4):423-8. Assay: Urea in mouse plasma.

Cavallini, N., et al. (2009). Substituting citrate for lactate in peritoneal dialysis fluid improves ultrafiltration in rats. Perit Dial Int 29(1):36-43. Assay: Urea in rat peritoneal dialysate.

Devalaraja-Narashimha, K., et al. (2009). Cyclophilin D gene ablation protects mice from ischemic renal injury. Am J Physiol Renal Physiol 297(3):F749-59. Assay: Urea in mouse blood.

Louie, A., et al. (2009). Pharmacodynamics of levofloxacin in a murine pneumonia model of Pseudomonas aeruginosa infection: determination of epithelial lining fluid targets. Antimicrob Agents Chemother 53(8):3325-30. Assay: Urea in mouse lung epithelial lining fluid.

Pesce, J.T., et al. (2009). Arginase-1-expressing macrophages suppress Th2 cytokine-driven inflammation and fibrosis. PLoS Pathog 5(4):e1000371. Assay: Urea in mouse macrophages.

Salerno, S., et al. (2009). Improved functions of human hepatocytes on NH3 plasma-grafted PEEK-WC-PU membranes. Biomaterials 30(26):4348-56. Assay: Urea in human primary hepatocytes.

Song, M.F., et al. (2009). Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG. Free Radic Biol Med 47(1):41-6. Assay: Urea in human urine.

Wang, A., et al. (2009). CXCR4/CXCL12 hyperexpression plays a pivotal role in the pathogenesis of lupus. J Immunol 182(7):4448-58. Assay: Urea in mouse blood.

Zafar, I., et al. (2009). Long-term rapamycin therapy in the Han:SPRD rat model of polycystic kidney disease (PKD). Nephrol Dial Transplant 24(8):2349-53. Assay: Urea in rat blood.

Costanzo, J.P., et al. (2008). Urea loading enhances postfreeze performance of frog skeletal muscle. J Comp Physiol B 178(3):413-20. Assay: Urea in frog muscle tissue.

Fairhurst, A.M., et al. (2008). Yaa autoimmune phenotypes are conferred by overexpression of TLR7. Eur J Immunol 38(7):1971-8. Assay: Urea in mouse blood.

Hattersley, S.M., et al. (2008). Development of a microfluidic device for the maintenance and interrogation of viable tissue biopsies. Lab Chip 8(11):1842-6. Assay: Urea in rat live tissue.

He, Z., et al. (2008). Interleukin-18 binding protein transgenic mice are protected against ischemic acute kidney injury. Am J Physiol Renal Physiol 295(5):F1414-21. Assay: Urea in mouse blood.

Meng, Q., et al. (2008). Regulating the age-related oxidative damage, mitochondrial integrity, and antioxidative enzyme activity in Fischer 344 rats by supplementation of the antioxidant epigallocatechin-3-gallate. Rejuvenation Res 11(3):649-60. Assay: Urea in rat plasma, urine.

Moertel, L., et al. (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Urea in Schistosoma japonicum protein extract; arginase activity.

Mols, M.Abee, T. (2008). Role of ureolytic activity in Bacillus cereus nitrogen metabolism and acid survival. Appl Environ Microbiol 74(8):2370-8. Assay: Urea in bacillus cereus medium.

Pauwels, F.E., et al. (2008). Evaluation of the diffusion of corticosteroids between the distal interphalangeal joint and navicular bursa in horses. Am J Vet Res 69(5):611-6. Assay: Urea in horse tissue.

Ramalingam, T.R., et al. (2008). Unique functions of the type II interleukin 4 receptor identified in mice lacking the interleukin 13 receptor alpha1 chain. Nat Immunol 9(1):25-33. Assay: Urea in mouse macrophage.

Yang, B., et al. (2008). Small-molecule CFTR inhibitors slow cyst growth in polycystic kidney disease. J Am Soc Nephrol 19(7):1300-10. Assay: Urea in mouse serum.

Botha, P., et al. (2007). Inhaled nitric oxide for modulation of ischemia-reperfusion injury in lung transplantation. J Heart Lung Transplant 26(11):1199-205. Assay: Urea in animal bronchoalveolar lavage.

Griffitt, R.J., et al. (2007). Exposure to copper nanoparticles causes gill injury and acute lethality in zebrafish (Danio rerio). Environ Sci Technol 41(23):8178-86. Assay: Urea in zebrafish plasma.

Horowitz, S., et al. (2007). Increased arginase activity and endothelial dysfunction in human inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol 292(5):G1323-36. Assay: Urea in human intestine tissue.

Levin, M.H., et al. (2007). Urearetics: a small molecule screen yields nanomolar potency inhibitors of urea transporter UT-B. FASEB J 21(2):551-63. Assay: Urea in dog MDCK kidney epithelium cell line.

Rodriguez-Iturbe, B., et al. (2007). Association of mitochondrial SOD deficiency with salt-sensitive hypertension and accelerated renal senescence. J Appl Physiol 102(1):255-60. Assay: Urea in mouse plasma.


To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.
Buy Now!!!
QuantiChrom™ Urea Assay Kit
Catalog No: DIUR-100
Price: $146    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: 4, -20°C
Urea Assay Kit

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