EnzyChrom™ Urea Assay Kit III (EUR3-100)
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative enzymatic determination of urea.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 50 to 1000 μM urea in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.

High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
OD340nm

Samples
Urea in biological samples (e.g. plasma, serum, urine, bronchoalveolar lavage (BAL)) and food/beverage samples (e.g. milk).

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
50 μM

Shelf Life
6 months

More Details
UREA, the major end product of protein catabolism in animals, is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for medical clinicians to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g. congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency, but may also result from over vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea or blood urea nitrogen (BUN) are popular in research and drug discovery.

BioAssay Systems' urea assay is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, α-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.

1. How do I store the kit?



This kit is shipped on ice. Upon receiving, please store the kit at -20°C. Urease can be stored at -20°C to 4°C.

2. Can I store unused reagents for future use?



Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. The dried NADH Reagent is stable for 3 weeks after reconstitution.

3. Does glutamate in the sample interfere with the assay?



High glutamate concentrations (> 2 mM) will interfere with the assay. For example a typical concentration of glutamate in cell culture medium is ~0.15 mM.

4. I do not see any color change in my wells. Is the assay not working?



This assay measures the decrease in OD at 340 nm and the color change is not visible to the naked eye.



For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
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EnzyChrom™ Urea Assay Kit III (EUR3-100)
Catalog No: EUR3-100
Price: $209    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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