QuantiChrom™ Urea Assay Kit II (DUR2-100)
- For quantitative enzymatic determination of urea.
- Fast and sensitive. Linear detection range (20 μL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
- Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk).
- 100 tests
- 1 mg/mL (0.17 mM)
- 6 months
- UREA, the major end product of protein catabolism in animals, is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for medical clinicians to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g. congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency, but may also result from over vigorous parenteral fluid therapy.
BioAssay Systems colorimetric urea (BUN) assay is based on urease catalyzed conversion of urea to ammonium and carbon dioxide. The pH of the reaction medium is monitored by a chromogen and the intensity of the reaction product at 557 nm is directly proportional to the urea concentration in the sample.
How do I store the kit?This kit is shipped at room temperature. Upon receiving, please store the Reagent and Standard in the refrigerator (4°C) and the Urease at -20°C to 4°C. For long term storage, keep the standard at -20°C.
There is a standard curve shown on the datasheet, why do I not construct one?Yes, there is a standard curve in the datasheet; we include this to show the linear detection range of the kit for quantifying urea. Due to interference, an internal standard must be run for most samples. The datasheet has detailed information on how to perform this and calculate urea concentration from it.
I would like to construct a standard curve, how can I go about that?If you wish to construct a standard curve, you may dilute the urea standard in dH2O and follow the instructions from question 4 below.
How can I detect urea in urine samples?DUR2-100 Urine Detection Protocol
Can I store unused reagents for future use?Yes, unused reagents can be stored according to the assay protocol.
I am not obtaining consistent data, what could be the problem?This assay is pH dependent; therefore, temperature can affect the results. Be sure to equilibrate all components to room temperature before each assay.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.