QuantiChrom™ TBARS Assay Kit
Protocol SDS

Application

  • For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation.

Key Features

  • Sensitive and accurate. Linear detection range: colorimetric assay 1 -30 μM, fluorometric assay 0.1 - 1.5 μM MDA.

Method

  • OD535nm, or FL530/550nm

Samples

  • Serum, plasma, urine, saliva etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.1 μM

Shelf Life

  • 6 months

More Details

  • Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. BioAssay Systems TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (λex/em = 530nm/550nm) is directly proportional to TBARS concentration in the sample.

What is the principle of the TBARS assay?

Biological specimens contain a mixture of thiobarbituric acid reactive substances (TBARS), including lipid hydroperoxides and aldehydes, which increase as a result of oxidative stress. Malondialdehyde (MDA) forms a 1:2 adduct with thiobarbituric acid. TBARS are expressed in terms of malondialdehyde (MDA) equivalents.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
KHAN, Z. J., KHAN, N. A., NASEEM, I., & NAMI, S. A. (2019). Indian Journal of Novel Drug Delivery. Indian Journal of Novel Drug Delivery, 11(1), 30-39. Assay: TBARS in rats blood.

Koner, D et al (2019). Antioxidant activity of endogenously produced nitric oxide against the zinc oxide nanoparticle-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur. Nitric Oxide 84:7-15. Assay: TBARS in catfish liver cells.

Pereira, B. A., Rocha, L. G. P., Teles, M. C., Silva, W. E., Barbosa, J. A., Rabelo, S. S. & Zangeronimo, M. G. (2019). Addition of chlorogenic acid and caffeine during the processing of cooled boar semen. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 71(2), 489-499. Assay: TBARS in boar plasma.

Yang, X., Kalchayanand, N., Belk, K. E., & Wheeler, T. L. (2019). Photohydroionization Reduces Shiga Toxin-Producing Escherichia coli and Salmonella on Fresh Beef with Minimal Effects on Meat Quality. Meat and Muscle Biology, 3(1), 105-115. Assay: TBARS in cow tissue.

Liang, K., Zhao, Y., Han, J., Liu, P., Qiu, J., Zhu, D. & Wang, X. (2018). Fatty acid composition, vitamin A content and oxidative stability of milk in China. Journal of Applied Animal Research, 46(1), 566-571. Assay: TBARS in cow milk.

Odhaib, K. J., Adeyemi, K. D., & Sazili, A. Q. (2018). Carcass traits, fatty acid composition, gene expression, oxidative stability and quality attributes of different muscles in Dorper lambs fed Nigella sativa seeds, Rosmarinus officinalis leaves and their combination. Asian-Australasian journal of animal sciences, 31(8), 1345. Assay: TBARS in lamb muscle tissue.

Laubenthal, L., Ruda, L., Sultana, N., Winkler, J., Rehage, J., Meyer, U. & Haussler, S. (2017). Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows. Journal of dairy science, 100(6), 4976-4986. Assay: TBARS in cow serum.

Morales-Gonzalez, A., Bautista, M., Mandrigal-Santillan, E., Posadas-Mondragon, A., Anguiano-Robledo, L., Mandrigal-Bajaidar, E. & Sanchez-Moreno, C. (2017). Nrf2 modulates cell proliferation and antioxidants defenses during liver regeneration induced by partial hepatectomy. Int. J. Clin. Exp. Pathol, 10, 7801-7811. Assay: TBARS in rats liver cells.

Adeyemi, K. D., Sabow, A. B., Aghwan, Z. A., Ebrahimi, M., Samsudin, A. A., Alimon, A. R., & Sazili, A. Q. (2016). Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend. Journal of animal science and technology, 58(1), 6. Assay: TBARS in goats serum.

Adeyemi, KD et al (2016). Effects of dietary oil blend on fatty acid composition, oxidative stability and physicochemical properties of Longissimus thoracis et lumborum muscle in goats. Animal Science Journal 87(11):1421-1432. Assay: TBARS in goat muscle cells.

Adeyemi, KD et al (2016).Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of Animal Science and Technology. 58:23. Assay: TBARS in goat muscle cells.

Soren, S., Singh, S. V., & Singh, P. (2016). Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions. Turkish Journal of Veterinary and Animal Sciences, 40(6), 797-802. Assay: TBARS in bulls plasma.

Teles, M. C., Pereira, B. A., Rocha, L. G. P., Resende, C. O., Rodrigues, V. V., Pereira, L. J. & Zangeronimo, M. G. (2016). Semen quality and reproductive performance of boars kept in pens containing conventional coffee husk as a floor covering. Revista Brasileira de Zootecnia, 45(7), 365-371. Assay: TBARS in pig plasma.

Al-Amran, F.G., et al. (2011). Amelioration of chronic cyclosporine A-induced nephrotoxicity by telmisartan in rats. African Journal of Pharmacy and Pharmacology 5(4):500-505. Assay: TBARS in rat renal tissue.

Davison, G. (2011). Innate immune responses to a single session of sprint interval training. Appl Physiol Nutr Metab 36(3):395-404. Assay: TBARS in human.

Shanmugasundaram, R.Selvaraj, R.K. (2011). Lutein supplementation alters inflammatory cytokine production and antioxidant status in F-line turkeys. Poult Sci 90(5):971-6. Assay: TBARS in turkey liver.

Verweij, M., et al. (2011). Preoperative fasting protects mice against hepatic ischemia/reperfusion injury: mechanisms and effects on liver regeneration. Liver Transpl 17(6):695-704. Assay: TBARS in mouse liver.

Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: TBARS in human blood.

Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: TBARS in Japanese quail blood.


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Please email or call 1-510-782-9988 x 2 to request assay service.
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QuantiChrom™ TBARS Assay Kit
Catalog No: DTBA-100
Price: $167    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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