QuantiChrom™ Sulfate Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism.

Key Features
Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min.

Method
OD600nm

Samples
Serum, urine, food and environment

Species
All

Procedure
5 min

Size
200 tests

Detection Limit
20 μM

Shelf Life
12 months

More Details
INORGANIC SULFATE is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics, steroids, neurotransmitters, and bile acids. Sulfate is needed for the biosynthesis of glycosaminoglycans, cerebroside sulfate, and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human inherited osteochon-drodysplasia disorders. In mammals, sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules, and only 5?20% of the filtered load is excreted into the urine. Simple, direct and automation-ready procedures for quantitative determination of inorganic sulfate find wide applications in research and drug discovery. BioAssay Systems sulfate assay kit is designed to measure sulfate concentration in biological fluids such as serum and urine. The improved method utilizes the quantitative formation of insoluble barium sulfate in polyethylene glycol. The turbidity measured between 540 and 610nm is proportional to sulfate level in the sample.

1. Why is a small amount of the standard added to the working reagent A.



This assay is based on the precipitation of insoluble barium sulfate which is measured turbidimetrically. Barium sulfate is minimally soluble, though, and to saturate the working reagent with soluble barium sulfate, you have to add a little of the sulfate standard to reagent A (which only contains barium). After that preparation step, all additional sulfate from your sample will precipitate when the working reagent is added allowing you to measure it.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Sedlak, L., Wojnar, W., Zych, M., Wygledowska-Promienska, D., Mrukwa-Kominek, E., & Kaczmarczyk-Sedlak, I. (2018). Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of rats with Streptozotocin-Induced Diabetes. Nutrients, 10(10), 1423. Assay: Sulfate in rat blood.

Tise, C. G., Anforth, L. E., Zhou, A. E., Perry, J. A., McArdle, P. F., Streeten, E. A. & Yerges-Armstrong, L. M. (2017). Sex-specific effects of serum sulfate level and SLC13A1 nonsense variants on DHEA homeostasis. Molecular genetics and metabolism reports, 10, 84-91. Assay: Sulfate in human serum.

Perry, C. G. (2016). A Macro View of a Micronutrient: Revealing the Genetics and Elucidating the Impact of Serum Sulfate on human Health in the Old Order Amish (Doctoral dissertation). Assay: Sulfate in human serum.

de Gouw, D., Hermans, P. W., Bootsma, H. J., Zomer, A., Heuvelman, K., Diavatopoulos, D. A., & Mooi, F. R. (2014). Differentially Expressed Genes in Bordetella pertussis Strains Belonging to a Lineage Which Recently Spread Globally. PloS one 9(1): e84523. Assay: Sulfate in B. pertussis cell culture medium.

Bose, A., Rogers, D. R., Adams, M. M., Joye, S. B., & Girguis, P. R. (2013). Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments. Frontiers in microbiology, 4. Assay: Sulfate in garden banks mud volcano site sediment.

Neff, M. W., Beck, J. S., Koeman, J. M., Boguslawski, E., Kefene, L., Borgman, A., & Ruhe, A. L. (2012). Partial deletion of the sulfate transporter SLC13A1 is associated with an osteochondrodysplasia in the Miniature Poodle breed. PloS one 7(12): e51917. Assay: Sulfate in miniature poodle blood, urine.

Reiser, V et al (2011). High-throughput simultaneous analysis of RNA, protein, and lipid biomarkers in heterogeneous tissue samples. Clinical chemistry 57(11): 1545-1555. Assay: Sulfate in human protein extract from plaque.

Wang, ZM et al (2011). Structural characterisation and immunomodulatory property of an acidic polysaccharide from mycelial culture of Cordyceps sinensis fungus Cs-HK1. Food Chemistry 125(2):637-43. Assay: Sulfate in fungi.

Ben Mansour, M., et al. (2010). Highly sulfated dermatan sulfate from the skin of the ray Raja montagui: anticoagulant activity and mechanism of action. Comp Biochem Physiol B Biochem Mol Biol 156(3):206-15. Assay: Sulfate in fish.

Sun X et al (2010). Deletion of the pH sensor GPR4 decreases renal acid excretion. J Am Soc Nephrol. 21(10):1745-55. Assay: Sulfate in mouse urine.

Ben Mansour, M., et al. (2009). Characterization of a novel dermatan sulfate with high antithrombin activity from ray skin (Raja radula). Thromb Res 123(6):887-94. Assay: Sulfate in fish.

Ben Mansour, M., et al. (2009). Polysaccharides from the skin of the ray Raja radula. Partial characterization and anticoagulant activity. Thromb Res 123(4):671-8. Assay: Sulfate in fish.

Majdoub, H., et al. (2009). Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis. Biochim Biophys Acta 1790(10):1377-81. Assay: Sulfate in Arthrospira platensis.

To find more recent publications, please click here.
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QuantiChrom™ Sulfate Assay Kit
Catalog No: DSFT-200
Price: $309    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: RT

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