EnzyChrom™ Sucrose Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of sucrose.

Key Features
No interference by glucose.

Use 20 μL samples. Linear detection range: 17 to 2000 μM sucrose.

Method
OD565nm

Samples
Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc

Species
All

Procedure
70 min

Size
100 tests

Detection Limit
17 μM

Shelf Life
6 months

More Details
Sucrose (C12H22O11) is a disaccharide of glucose and fructose with a 1,2-glycosidic linkage. It is the most common food sweetener and the most important sugar in plants. In mammals, sucrose is readily digested in the stomach into glucose and fructose, which are rapidly absorbed into the bloodstream in the small intestine. Simple, direct and high-throughput assays for measuring sucrose concentrations find wide applications. BioAssay Systems improved assay uses invertase to digest sucrose into fructose and glucose. The resulting fructose is then quantified using our fructose assay reagent. The measured color intensity at 565nm is directly proportional to the sucrose concentration in the sample.
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Chen, X., Zhu, M., Gu, F., Liu, M., Zhang, Y., Xing, Y., & He, G. (2018). Identification and gene fine mapping of starch accumulation and early senescent leaf mutant esl10 in rice. Crop Science, 58(1), 204-217. Assay: Sucrose in microbe leaves.

Huang, J., Yan, M., Zhu, X., Zhang, T., Shen, W., Yu, P., & He, G. (2018). Gene mapping of starch accumulation and premature leaf senescence in the ossac3 mutant of rice. Euphytica, 214(10), 177. Assay: Sucrose in e. coli leaves.

Inukai, T. (2017). Differential Regulation of Starch-synthetic Gene Expression in Endosperm Between Indica and Japonica Rice Cultivars. Rice, 10(1), 7. Assay: Sucrose in rice karyopsis tissue.

Nakajima, Y., Maeda, K., Jin, Q., Takahashi-Ando, N., Kanamaru, K., Kobayashi, T., & Kimura, M. (2016). Oligosaccharides containing an alpha-( - 2)(glucosyl/xylosyl)-fructosyl linkage as inducer molecules of trichothecene biosynthesis for Fusarium graminearum. International journal of food microbiology, 238, 215-221. Assay: Sucrose in Fusarium graminearum cell lysate.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Sucrose Assay Kit
Catalog No: ESUC-100
Price: $339    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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