EnzyChrom™ Starch Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of starch and evaluation of drug effects on starch metabolism.

Key Features
Use as little as 10 μL samples. Linear detection range: 2 to 200 μg/mL starch for colorimetric assays and 0.2 to 20 μg/mL for fluorimetric assays.

Method
OD570nm, or FL530/585nm

Samples
Biological, agriculture, food etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
2 μg/mL

Shelf Life
6 months

More Details
STARCH , chemical formula (C6H10O5)n, is a polysaccharide carbohydrate consisting of a large number of glucose units joined together by glycosidic bonds. All plant seeds and tubers contain starch present in the form of amylose and amylopectin. Starch is the most consumed polysaccharide in the human diet. Some starches are digested very quickly, and cause a rapid and large rise in blood sugar. Others are digested more slowly, and some starch, called resistant starch, is not digested in the small intestine at all, and thus causes little or no blood sugar rise. Simple, direct and automation-ready procedures for measuring starch concentrations find wide applications in research and drug discovery. BioAssay Systems starch uses a single Working Reagent that combines the enzymatic break down of starch and the detection of glucose in one step. The color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the starch concentration in the sample. This simple convenient assay is carried out at room temperature and takes only 30 min.

1. What is the principle of the starch assay?



Starch is first broken down into glucose molecules by α-amylase and amyloglucosidase. Next, glucose is converted to glucuronic acid and H2O2 by the enzyme glucose oxidase. Finally, H2O2 and ADHP are converted to the pink colored product resorufin and water by horse radish peroxidase.

2. What is the difference between soluble and resistant starch?



Soluble starch is starch that is soluble when heated to 60°C. Resistant starch does not dissolve

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
South, P. F., Cavanagh, A. P., Liu, H. W., & Ort, D. R. (2019). Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field. Science, 363(6422), eaat9077. Assay: Starch in plant seed.

Ort, D. R., South, P. F., & Walker, B. (2018). U.S. Patent Application No. 15/913,395. Assay: Starch in Nicotiana tabacum leaf.

Wilson, C. M. (2016). Long-term impacts of invasive herbivores on tree physiology, growth, and phenology: A whole-tree perspective. Assay: Starch in hemlock root.

Gardner RD et al (2013). Comparison of CO(2) and bicarbonate as inorganic carbon sources for triacylglycerol and starch accumulation in Chlamydomonas reinhardtii. Biotechnol Bioeng. 110(1):87-96. Assay: Starch in algae Chlamydomonas reinhardtii.

Tsuge, Y., Tateno, T., Sasaki, K., Hasunuma, T., Tanaka, T., & Kondo, A. (2013). Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions. AMB Express 3(1): 72. Assay: Starch in Corynebacterium glutamicum cell culture.

Boriboonkaset T et al (2012) Expression levels of some starch metabolism related genes in flag leaf of two contrasting rice genotypes exposed to salt stress. Aust. J. Crop Sci. 6(11):1579-1586. Assay: Starch in plant rice leaf.

Boriboonkaset T et al (2012) Expression levels of some starch metabolism related genes in flag leaf of two contrasting rice genotypes exposed to salt stress. Aust. J. Crop Sci. 6(11):1579-1586. Assay: Starch in Plant Rice leaf.

Zebeli Q et al (2011) Intraruminal administration of Megasphaera elsdenii modulated rumen fermentation profile in mid-lactation dairy cows. J Dairy Res 13:1-10. Assay: Starch in plant cow feed.

Iqbal S et al (2009) Feeding barley grain steeped in lactic acid modulates rumen fermentation patterns and increases milk fat content in dairy cows. J Dairy Res 92(12):6023-32. Assay: Starch in food grain starch.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Starch Assay Kit
Catalog No: E2ST-100
Price: $319    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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