QuantiChrom™ Sorbitol Dehydrogenase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of sorbitol dehydrogenase enzyme activity.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 0.1 to 125 U/L for 12 min reaction.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD565nm

Samples
Biological samples e.g. plasma, serum, urine, tissue and culture media.

Species
All

Procedure
15 min

Size
100 tests

Detection Limit
0.1 U/L

Shelf Life
6 months

More Details
SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. BioAssay Systems' non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
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QuantiChrom™ Sorbitol Dehydrogenase Assay Kit
Catalog No: DSDH-100
Price: $369    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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