EnzyChrom™ Sorbitol Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 5 to 1000 μM D-sorbitol.

Convenient and high-throughput. Room temperature assay. No 37°C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD565nm

Samples
Biological (e.g. blood), food, beverage and agriculture samples.

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
5 μM

Shelf Life
6 months

More Details
SORBITOL (glucitol) is a sugar alcohol that is metabolized slowly in the human body. Sorbitol can be obtained from glucose by reducing aldehyde group to a hydroxyl group. Accumulation of excessive sorbitol in erythrocytes, retinal cells, and Schwann cells has been associated with retinopathy, cataracts, peripheral neuropathy and diabetes. Sorbitol is made solely from corn syrup, and found in fruits such as apples, pears, peaches, and prunes. It is widely used as a sugar substitute and as a laxative. It is also utilized in specialty culture media and in healthcare, food and cosmetic products. Sorbitol is measured in biological samples to monitor metabolic pathways and the progression of diabetes. BioAssay Systems sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the sorbitol concentration.
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Sedlak, L et al (2018). Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of rats with Streptozotocin-Induced Diabetes. Nutrients, 10(10), 1423. Assay: Sorbitol in rat cell lysate, lens.

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EnzyChrom™ Sorbitol Assay Kit
Catalog No: ESBT-100
Price: $369    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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