QuantiChrom™ Sialic Acid Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid.

Key Features
Sensitive and accurate. Use as little as 60 μL samples. Linear detection range in 96-well plate: 5 to 1000 μM sialic acid for colorimetric assays and 0.5 to 100 μM for fluorimetric assays.

Method
OD549nm, or FL555/585nm

Samples
Serum, plasma, saliva, milk etc

Species
All

Procedure
2.5 hrs

Size
100 tests

Detection Limit
OD, FL: 5, 0.5 μM

Shelf Life
12 months

More Details
SIALIC ACID is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple and direct procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. BioAssay Systems’ sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink colored product. The color intensity at 549 nm or fluorescence intensity at λex/em = 555/585 nm is directly proportional to sialic acid concentration in the sample.

1. What is the difference between your two sialic acid assay kits?



The DSLA-100 assay kit is based on the oxidation of sialic acid with periodate and detection of the reaction product formyl pyruvic acid with thiobarbituric acid.The ESLA-100 assay kit is based on enzymatic digestion of sialic acid with NANA-aldolase (N-acetyl-neuraminic acid aldolase) and detection of the breakdown product pyruvate.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Carvalho FA et al (2011). Variations on fibrinogen-erythrocyte interactions during cell aging. PLoS One. 6(3):e18167. Assay: Sialic Acid in Human blood (Pubmed).

2. Chrostek, L et al (2013). Sialic acid levels reflects the disturbances of glycosylation and acute-phase reaction in rheumatic diseases. Rheumatology International. 34(3): 393-399. Assay: Serum in Humans (Pubmed).

3. Pawluczyk, I. Z., Ghaderi Najafabadi, M., Patel, S., Desai, P., Vashi, D., Saleem, M. A., & Topham, P. S. (2014). Sialic acid attenuates puromycin aminonucleoside-induced desialylation and oxidative stress in human podocytes. Experimental cell research 320(2): 258-268. Assay: Sialic Acid in Human podocyte (Pubmed).

4. Gantner, M et all (2014). Partial synthesis of ganglioside and lysoganglioside lipoforms as internal standards for MS quantification. Journal of Lipid Research. 55, 2692-2704. Assay: Brain Gangliosides in Bovines (Pubmed).

5. Taib, I.S. et al.(2015) "Palm oil tocotrienol-rich fraction attenuates testicular toxicity induced by fenitrothion via an oxidative stress mechanism." Toxicology Research 4.1: 132-142. Assay: Sialic Acid in Rat tissue.

To find more recent publications, please click here.
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QuantiChrom™ Sialic Acid Assay Kit
Catalog No: DSLA-100
Price: $389    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: RT, -20°C



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