EnzyChrom™ Phospholipid Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

Key Features
Sensitive. Use 20 μL samples. Linear detection range: colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid.

Method
OD570nm, or FL530/585nm

Samples
Biological

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
OD, FL: 3, 0.6 μM

Shelf Life
6 months

More Details
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

1. I am looking for assay to measure phospholipids in cell culture media. I think the concentration in media maybe much lower than the serum. I am not sure whether if this kit is suitable for me.



BioAssay Systems’ phospholipid assay kit has a linear detection range from 3 - 200 μM (colorimetric assay), and 0.6 - 20 μM (fluorimetric assay). We have not tested cell culture media, but believe it should work in most cases. Common media formulations will contain about 5-10% FCS and the phospholipid content therein should be in the linear detection range of the assay. Other media formulations may have much lower serum concentrations or are serum-free. In those cases you should contact the manufacturer to get an estimate of how much phospholipid is in your medium.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Dassuncao, C., Pickard, H., Pfohl, M., Tokranov, A. K., Li, M., Mikkelsen, B., & Sunderland, E. M. (2019). Phospholipid Levels Predict the Tissue Distribution of Poly-and Perfluoroalkyl Substances in a Marine Mammal. Environ Sci Tech Let, 6(3), 119-125. Assay: Phospholipid in human tissues.

Siew, S. M., Cunningham, S. C., Zhu, E., Tay, S. S., Venuti, E., Bolitho, C., & Alexander, I. E. (2019). Prevention of cholestatic liver disease and reduced tumorigenicity in a murine model of PFIC type 3 using hybrid AAV-piggyBac gene therapy. Hepatology, doi:10.1002/hep.30773 Assay: Phospholipid in rat Liver tissue.

Badylak, S. F., & Keane Jr, T. J. (2018). Methods of preparing ecm scaffolds and hydrogels from colon. U.S. Patent Application No. 15/742,676. Assay: Phospholipid in human intestinal epithelial cells.

Bhattacharjee, P., & Keyel, P. A. (2018). Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Scientific reports, 8(1), 6458. Assay: Phospholipid in murine bone marrow derived macrophages.

Alexander, I., Cunningham, S., & Nagy, A. (2017). Stable Gene Transfer to Proliferating Cells. U.S. Patent Application No. 15/127,743. Assay: Phospholipid in monkey bile.

Keane, T. J., Dziki, J., Castelton, A., Faulk, D. M., Messerschmidt, V., Londono, R., & Badylak, S. F. (2017). Preparation and characterization of a biologic scaffold and hydrogel derived from colonic mucosa. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 105(2), 291-306. Assay: Phospholipid in pig colon.

Yang, W., Huang, L., Gao, J., Wen, S., Tai, Y., Chen, M., & Li, J. (2017). Betaine attenuates chronic alcohol-induced fatty liver by broadly regulating hepatic lipid metabolism. Molecular medicine reports, 16(4), 5225-5234. Assay: Phospholipid in rat hepatocytes, serum.

Jeong, J., Kim, J., Seok, S. H., & Cho, W. S. (2016). Indium oxide (In 2 O 3) nanoparticles induce progressive lung injury distinct from lung injuries by copper oxide (CuO) and nickel oxide (NiO) nanoparticles. Archives of toxicology, 90(4), 817-828. Assay: Phospholipid in mouse.

Dhamdhere, GR et al (2014). Drugging a stem cell compartment using Wnt3a protein as a therapeutic. PloS one 9.1: e83650. Assay: Phospholipid in synthetic liposome.

Goktas, S et al (2014). Decellularization method influences early remodeling of an allogenic tissue scaffold. Journal of Biomedical Materials Research Part A102.1: 8-16. Assay: Phospholipid in human umbilical cord tissue.

Tan, LN et al (2014). Liquid crystal droplet-based amplification of microvesicles that are shed by mammalian cells. Analyst. Assay: Phospholipid in human epidermoid cancer cell line.

Gong, J et al (2013). Microparticle drug sequestration provides a parallel pathway in the acquisition of cancer drug resistance. European journal of pharmacology 721.1: 116-125. Assay: Phospholipid in synthetic microparticles.

Kaszas, N et al (2013). Ciprofloxacin encapsulation into giant unilamellar vesicles: Membrane binding and release. Journal of pharmaceutical sciences 102.2: 694-705. Assay: Phospholipid in synthetic vesicles.

Haynes TA et al (2012). DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta 1821(7):943-53. Assay: Phospholipid in human Ovarian cancer cells.

Kim S et al (2012) Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol 22(1):41-47. Assay: Phospholipid in lipid.

Mukherjee, A et al (2012). Lysophosphatidic acid activates lipogenic pathways and de novo lipid synthesis in ovarian cancer cells. Journal of Biological Chemistry 287.30: 24990-25000. Assay: Phospholipid in human ovarian cancer cell line.

Soga N et al (2012) Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis. J Biol Chem 287(12):9633-9. Assay: Phospholipid in human cancer cell.

Stoll C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-481. Assay: Phospholipid in mouse brain.

Stoll C, et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-81. Assay: Phospholipid in human red blood cell.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Phospholipid Assay Kit
Catalog No: EPLP-100
Price: $359    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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