EnzyChrom™ Phospholipid Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

Key Features
Sensitive. Use 20 μL samples. Linear detection range: colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid.

Method
OD570nm, or FL530/585nm

Samples
Biological

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
OD, FL: 3, 0.6 μM

Shelf Life
6 months

More Details
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

1. I am looking for assay to measure phospholipids in cell culture media. I think the concentration in media maybe much lower than the serum. I am not sure whether if this kit is suitable for me.



BioAssay Systems’ phospholipid assay kit has a linear detection range from 3 - 200 μM (colorimetric assay), and 0.6 - 20 μM (fluorimetric assay). We have not tested cell culture media, but believe it should work in most cases. Common media formulations will contain about 5-10% FCS and the phospholipid content therein should be in the linear detection range of the assay. Other media formulations may have much lower serum concentrations or are serum-free. In those cases you should contact the manufacturer to get an estimate of how much phospholipid is in your medium.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions

1. Methods for treatment of a sarcoma using an epimetabolic shifter (Coenzyme q10. US 2011/0064747. Assay: Phospholipid in Human Red blood cell (Pubmed).

2. Kim S et al (2012) Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol 22(1):41-47. Assay: Phospholipid in N/A lipid (Pubmed).

3. Soga N et al (2012) Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis. J Biol Chem 287(12):9633-9. Assay: Phospholipid in human cancer cell (Pubmed).

4. Haynes TA et al (2012) DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta 1821(7):943-53. Assay: Phospholipid in Human Ovarian cancer cells (Pubmed).

5. Stoll C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-481. Assay: Phospholipid in Mouse Brain (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Phospholipid Assay Kit
Catalog No: EPLP-100
Price: $339    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C



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