PiBlue™ Phosphate Assay Kit
Datasheet PDF  MSDS/Bulk Order

For sensitive and high-throughput phosphate determination.

Key Features
Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits.

High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 μM and 50 μM phosphate.

Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes.

Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers.

Robust and amenable to HTS: Z’ factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems

OD620nm (malachite green)

Enzyme reactions with free phosphate releases


30 min

500 tests

Detection Limit
0.4 μM

Shelf Life
12 months

More Details
The PiBlue™ Phosphate Assay Kit is based on a proprietary formulation of the malachite green dye. The PiBlue™ reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high-throughput screening of enzyme inhibitors.
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1. Lau KH et al (2006). An osteoclastic protein-tyrosine phosphatase is a potential positive regulator of the c-Src protein-tyrosine kinase activity: a mediator of osteoclast activity. J Cell Biochem. 97(5):940-55. Assay: Phosphate in Mouse cytosolic fraction (Pubmed).

2. Aldana-Masangkay GI et al (2011). Tubacin suppresses proliferation and induces apoptosis of acute lymphoblastic leukemia cells. Leuk Lymphoma. 52(8):1544-55. Assay: Phosphate in Rabbit phosphatases (Pubmed).

3. Liu Q et al (2011). Competition between foliar Neotyphodium lolii endophytes and mycorrhizal Glomus spp. fungi in Lolium perenne depends on resource supply and host carbohydrate content. Functional Ecology 25:910–920. Assay: Phosphate in Mouse intracellular phosphate (Pubmed).

4. Chen X et al (2007). The isolation and structure of membrane lipid rafts from rat brain. Biochimie 89(2):192-6. Assay: Phosphate in mouse/human cellular extract (Pubmed).

5. Hough TA et al (2007). Novel mouse model of autosomal semidominant adult ypophosphatasia has a splice site mutation in the tissue nonspecific alkaline phosphatase gene Akp2. J Bone Miner Res. 22(9):1397-407. Assay: Phosphate in Frog Na+/K+-ATPase extract (Pubmed).

6. Kang HY et al (2008). Altered TNSALP expression and phosphate regulation contribute to reduced mineralization in mice lacking androgen receptor. Mol Cell Biol.28(24):7354-67. Assay: Phosphate in Rat ATPase extract (Pubmed).

7. Cramp RL et al (2009). Ups and downs of intestinal function with prolonged fasting during aestivation in the burrowing frog, Cyclorana alboguttata. J Exp Biol. 212(22):3656-63. Assay: Phosphate in Mouse lipid Pi (Pubmed).

8. Demidenko AA et al (2011). Effects of viscogens on RNA transcription inside reovirus particles. J Biol Chem. 2011 Jun 30. Assay: Phosphate in Rat lipid (Pubmed).

9. Polewski MD et al (2010). Inorganic pyrophosphatase induces type I collagen in osteoblasts. Bone 46(1):81-90. Assay: Phosphate in Human blood, semen (Pubmed).

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PiBlue™ Phosphate Assay Kit
Catalog No: POPB-500
Price: $169    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4°C

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