QuantiChrom™ Peroxidase Assay Kit (D2PD-100)
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of peroxidase activity.

Key Features
Fast and sensitive. Use as little as 10 μL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD570nm, or FL530/585nm

Samples
Biological

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
OD, FL: 4, 0.8 U/L

Shelf Life
12 months

More Details
PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
ROOR + electron donor (2 e-) + 2H+ → ROH + R’OH.
For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase). Simple, direct and automation-ready procedures for determining peroxidase activity find wide applications. BioAssay Systems peroxidase assay uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λex/em = 530/590nm) is a direct measure of the enzyme activity.duangwatch
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Ismail, I. B., Al-Busadah, K. A., & El-Bahr, S. M. (2013). Oxidative stress biomarkers and biochemical profile in broilers chicken fed zinc bacitracin and ascorbic acid under hot climate. Am J Biochem Mol Biol, 3, 202-214. Assay: Peroxidase in broiler chicks liver cells.

Hokari, R., et al. (2011). Increased expression of lipocalin-type-prostaglandin D synthase in ulcerative colitis and exacerbating role in murine colitis. Am J Physiol Gastrointest Liver Physiol 300(3):G401-8. Assay: Peroxidase in mouse.

Jawed H et al (2010) N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5 Assay: Peroxidase in rat serum.

Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther.

Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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QuantiChrom™ Peroxidase Assay Kit (D2PD-100)
Catalog No: D2PD-100
Price: $279    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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