Parallel Artificial Membrane Permeability Assay-BBB Kit
- Assess blood brain barrier (BBB) membrane permeability of test compounds.
- Convenient. Includes all necessary equipment to run a PAMPA plate.
- Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
- Chemical compounds
- 96 tests
- 12 months
- MEMBRANE PERMEABILITY is an important characteristic to determine for evaluating compounds as potential drug candidates. Drugs often need to cross cell membranes in order to reach their target of action and this makes a compound’s ability to passively cross these membranes an important characteristic to evaluate. The Blood Brain Barrier (BBB) is made of brain endothelial cells with tight junctions. Rapid and early screening of compounds for BBB penetration is highly desirable for drug discovery. Permeability can be evaluated by cell-based methods; however, these methods are often expensive and time consuming. Parallel Artificial Permeability Assays (PAMPA) offer researchers a quick, inexpensive method of evaluating the permeability of test compounds. Our PMBBB-096 kit is designed to aid in evaluating BBB permeability. BioAssay Systems' PMBBB Kit provides all the necessary components to run a Parallel Artificial Permeability Assay for Blood Brain Barrier studies.
What are the Permeability Controls?The High, Medium, and Low Controls are 10 mM solutions of Promazine Hydrochloride, Clonidine, and Diclofenac, respectively, in DMSO.
What other materials are required to run the assay?Pipetting devices, DMSO, PBS, UV Plates (Cat # P96UV), and an absorbance plate reader capable of absorbance spectra.
What is the membrane of the donor plate?The donor plate membrane is a Hydrophobic PVDF membrane with a pore size of 0.45 µm.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.