EnzyChrom™ Nitric Oxide Synthase Assay Kit
ProtocolSDS


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Application
For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.

Key Features
Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate.

Rapid and reliable. Can be completed in 40 min if reduction of NO3- to NO2- is performed at 60°C.

Method
OD540nm

Samples
Biological

Species
All

Procedure
40 min

Size
100 tests

Detection Limit
0.25 U/L

Shelf Life
6 months

More Details
Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in Research and Drug Discovery. BioAssay Systems EnzyChrom™ Nitric Oxide Synthase Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 min.
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Meng, JP (2013). Hydrogen sulfide promotes nitric oxide production in corpus cavernosum by enhancing expression of endothelial nitric oxide synthase. International journal of impotence research 25.3: 86-90. Assay: Nitric Oxide Synthase in Rat Corpus Cavernosum Tissue (Pubmed).

2. Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Tox. & Environ. Chem. 96(2):307-317 Assay: Nitric Oxide Synthase in Rat tissue (Pubmed).

3. Gehman, MA (2015). The role of BATF2 in LPS/IFN? polarized macrophages. Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics 10: 1-205.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Nitric Oxide Synthase Assay Kit
Catalog No: ENOS-100
Price: $369    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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