EnzyChrom™ Neuraminidase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor.

Key Features
Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays.

Simple and convenient. Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay to screen thousands of samples per day.

Method
OD570nm, or FL530/585nm

Samples
Biological

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
OD, FL: 1, 0.01U/L

Shelf Life
6 months

More Details
NEURAMINDASE (also known as Sialidase) is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery. BioAssay Systems neuraminidase assay measures the sialic acid released by neuraminidase in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to neuraminidase activity in the sample.

1. Could you tell me the chemical components of the substrate? What kind of sialic acid?



The substrate is fetuin, a heavily sialidated protein from fetal bovine serum. Our assay is based on the detection of N-acetyl neuraminic acid (NANA, Neu5Ac) in a coupled enzyme reaction leading to the formation of a colored and fluorescent product. Influenza neuraminidase will cleave terminal NANA residues from fetuin.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Subathra, M et al (2014). Evaluation of antibody response in mice against avian influenza A (H5N1) strain neuraminidase expressed in yeast Pichia pastoris. Journal of Biosciences: 1-9. Assay: Neuraminidase in Yeast Purified Lysate.

2. Li, J., et al. (2013) CodY is a global regulator of virulence-associated properties for. Clostridium perfringens: 00770-13. Assay: Neuraminidase in C. perfringens Cells (Pubmed).

3. Subathra, M et al (2014). Evaluation of antibody response in mice against avian influenza A (H5N1) strain neuraminidase expressed in yeast Pichia pastoris. J Biosci. 39(3):443-51. Assay: Neuraminidase in Pichia pastoris cells (Pubmed).

4. Jihong L, et al (2014). The Sialidases of Clostridium perfringens Type D Strain CN3718 Differ in Their Properties and Sensitivities to Inhibitors. Appl Environ Microbiol. 80(5): 1701?1709. Assay: Neuraminidase in Clostridium perfringens cells (Pubmed).

5. Itoh, Y et al (2015). Emergence of H7N9 Influenza A Virus Resistant to Neuraminidase Inhibitors in Nonhuman Primates. Antimicrob Agents Chemotherapy. 59(8):4962-73". Assay: Fluid Samples in Macaque (Pubmed).

To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please inquire via email or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyChrom™ Neuraminidase Assay Kit
Catalog No: ENEU-100
Price: $439    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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