EnzyFluo™ NFKB Phosphorylation Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.

Key Features
Simple and convenient. No cell lysis necessary, cells can be cultured for several days.

Method
FL360/450nm, 530/585nm

Samples
Cells

Species
Human, mouse

Procedure
8 hrs

Size
100 tests

Shelf Life
6 months

More Details
Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. BioAssay Systems cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.
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Maniam, P., et al. (2015). Regulatory role of GSK3beta in the activation of NF-kB and modulation of cytokine levels in Burkholderia pseudomallei-infected PBMC isolated from streptozotocin-induced diabetic animals. Tropical biomedicine, 32(1), 36-48. Assay: NFKB phosphorylation in rat blood cell (PBMC).

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EnzyFluo™ NFKB Phosphorylation Assay Kit
Catalog No: ENFKB-100
Price: $459    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

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Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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