EnzyFluo™ NFKB Phosphorylation Assay Kit
Protocol
SDS
Application
- For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.
Key Features
- Simple and convenient. No cell lysis necessary, cells can be cultured for several days.
Method
- FL360/450nm, 530/585nm
Samples
- Cells
Species
- Human, mouse
Size
- 100 tests
Shelf Life
- 6 months
More Details
- Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. BioAssay Systems cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.
Haven’t Found the ELISA Kit for Your Target?
We offer a DIY kit. All that is required is a primary antibody.
If you prefer, we can develop the assay for you. Please Email or call 510-782-9988x2.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Maniam, P., et al. (2015). Regulatory role of GSK3beta in the activation of NF-kB and modulation of cytokine levels in Burkholderia pseudomallei-infected PBMC isolated from streptozotocin-induced diabetic animals. Tropical biomedicine, 32(1), 36-48. Assay: NFKB phosphorylation in rat blood cell (PBMC).
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