EnzyFluo™ NFKB Phosphorylation Assay Kit
Protocol SDS Printed Protocols and SDSs are not provided with kits.
Application
• For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.
Key Features
• Simple and convenient. No cell lysis necessary, cells can be cultured for several days.
Method
• FL360/450nm, 530/585nm
Samples
• Cells
Species
• Human, mouse
Procedure
• 8 hrs
Size
• 100 tests
Shelf Life
• 6 months
More Details
• Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. BioAssay Systems cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.
Application
• For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.
Key Features
• Simple and convenient. No cell lysis necessary, cells can be cultured for several days.
Method
• FL360/450nm, 530/585nm
Samples
• Cells
Species
• Human, mouse
Procedure
• 8 hrs
Size
• 100 tests
Shelf Life
• 6 months
More Details
• Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. BioAssay Systems cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Maniam, P., et al. (2015). Regulatory role of GSK3beta in the activation of NF-kB and modulation of cytokine levels in Burkholderia pseudomallei-infected PBMC isolated from streptozotocin-induced diabetic animals. Tropical biomedicine, 32(1), 36-48. Assay: NFKB phosphorylation in rat blood cell (PBMC).
To find more recent publications, please click here.
To find more recent publications, please click here.
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Technical support provided by the very scientists that develop the assays.
Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market
Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.
Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.
Technical support provided by the very scientists that develop the assays.
Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market
Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.
Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.
BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers
