EnzyFluo™ NADP/NADPH Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Key Features
Sensitive and accurate. Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
FL530/585nm

Samples
Cell, tissue extracts etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
0.01 μM

Shelf Life
6 months

More Details
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems’ EnzyFluo™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient method to measure NADP+, NADPH and their ratio.

1. When tissue is used, should it be freshly obtained? Or is -80°C storage ok?



If direct sample processing is not possible, we recommend snap freezing tissue samples in liquid nitrogen and keeping them either in liquid nitrogen or at -80°C until further processing.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
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EnzyFluo™ NADP/NADPH Assay Kit
Catalog No: EFNP-100
Price: $349    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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