EnzyFluo™ NADP/NADPH Assay Kit
Protocol SDS

Application

  • For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Key Features

  • Sensitive and accurate. Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

  • FL530/585nm

Samples

  • Cell, tissue extracts etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.01 μM

Shelf Life

  • 6 months

More Details

  • Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems EnzyFluo™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient method to measure NADP+, NADPH and their ratio.

When tissue is used, should it be freshly obtained? Or is -80°C storage ok?

If direct sample processing is not possible, we recommend snap freezing tissue samples in liquid nitrogen and keeping them either in liquid nitrogen or at -80°C until further processing.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Li, Xiao-xue, et al (2018). Nuclear receptor Nur77 facilitates melanoma cell survival under metabolic stress by protecting fatty acid oxidation. Molecular cell 69.3: 480-492. Assay: NADP/NADPH in mice melanoma.

Guo, Shuang, et al. (2015) Resveratrol attenuates high glucose-induced oxidative stress and cardiomyocyte apoptosis through AMPK. Molecular and cellular endocrinology 412: 85-94. Assay: NADP/NADPH in rat cardiac cells.

Zhu, Lingfeng, et al. (2015) NADP+-preferring D-lactate dehydrogenase from Sporolactobacillus inulinus. Applied and environmental microbiology 81.18: 6294-6301. Assay: NADP/NADPH in e. coli recombinant plasmid.


To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.
Buy Now!!!
EnzyFluo™ NADP/NADPH Assay Kit
Catalog No: EFNP-100
Price: $379    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.