EnzyChrom™ NADP/NADPH Assay Kit

EnzyChrom™ NADP/NADPH Assay Kit
Catalog No: ECNP-100
Price: $429    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment:Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C
NADP/NADPH Assay Kit (ECNP-100)
Protocol SDS


  • For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Key Features

  • Sensitive and accurate. Detection limit 0.1 μM, linearity up to 10 μM NADP+/NADPH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.


  • OD565nm


  • Cell, tissue extracts etc


  • All


  • 100 tests

Detection Limit

  • 0.1 μM

Shelf Life

  • 6 months

More Details

  • Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems EnzyChrom™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.

When tissue is used, should it be freshly obtained? Or is -80°C storage ok?

If direct sample processing is not possible, we recommend snap freezing tissue samples in liquid nitrogen and keeping them either in liquid nitrogen or at -80°C until further processing.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Satowa, D., et al. (2020). Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl-CoA supply. Biotechnology and Bioengineering 117(7): 2153-2164. Assay: NADP and NADPH in E. coli cells.

Tokuda, K., et al. (2019). Up-regulation of the pentose phosphate pathway and HIF-1? expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina. Cell Biology International 44(1): 137-144. Assay: NADP and NADPH in rat tissue.

Cao, C.-H., et al. (2021). Enzyme cascade for biocatalytic deracemization of D,L-phosphinothricin. Journal of Biotechnology 325: 372-379. Assay: NADP and NADPH in.

Shen, B., et al. (2019). Necrostatin-1 attenuates renal ischemia and reperfusion injury via meditation of HIF-1?/mir-26a/TRPC6/PARP1 signaling. Molecular Therapy. Nucleic Acids 17: 701-713. Assay: NADP and NADPH in human cells.

Noh, M. R., et al. (2020). Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension. Redox Biology 34: 101548. Assay: NADP and NADPH in mouse renal tissue mitochondrial fraction.

Cao, L., et al. (2021). Cryptotanshinone strengthens the effect of gefitinib against non-small cell lung cancer through inhibiting transketolase. European Journal of Pharmacology 890: 173647. Assay: NADP and NADPH in cells.

Zhu, J., et al. (2020). Exogenous NADPH ameliorates myocardial ischemia-reperfusion injury in rats through activating AMPK/mTOR pathway. Acta Pharmacologica Sinica 41(4): 535-545. Assay: NADP and NADPH in rat plasma and myocardium.

Sato, N., et al. (2020). Metabolic engineering of shikimic acid-producing Corynebacterium glutamicum from glucose and cellobiose retaining its phosphotransferase system function and pyruvate kinase activities. Frontiers in Bioengineering and Biotechnology 8: 569406. Assay: NADP and NADPH in C. glutamicum cells.

Kwak, S., et al. (2020). Redirection of the glycolytic flux enhances isoprenoid production in Saccharomyces cerevisiae. Biotechnology Journal 15(2): e1900173. Assay: NADP and NADPH in yeast cells.

Zhang, Y., et al. (2021). High-level production of l-homoserine using a non-induced, non-auxotrophic Escherichia coli chassis through metabolic engineering. Bioresource Technology 327: 124814. Assay: NADP and NADPH in E. coli.

Gao, X., et al. (2019). ?-6-phosphogluconolactone, a byproduct of the oxidative pentose phosphate pathway, contributes to AMPK activation through inhibition of PP2A. Molecular Cell 76(6): 857-871.e9. Assay: NADP and NADPH in human cells.

Zhang, Y., et al. (2021). Hepatic stellate cells specific liposomes with the Toll-like receptor 4 shRNA attenuates liver fibrosis. Journal of Cellular and Molecular Medicine 25(2): 1299-1313. Assay: NADP and NADPH in mouse liver tissue.

Wu, C.-K., et al. (2021). Renal tubular epithelial TRPA1 acts as an oxidative stress sensor to mediate ischemia-reperfusion-induced kidney injury through MAPKs/NF-?B signaling. International Journal of Molecular Sciences 22(5). Assay: NADP and NADPH in human cells.

Ko, H.-K., et al. (2020). Lung epithelial TRPA1 mediates lipopolysaccharide-induced lung inflammation in bronchial epithelial cells and mice. Frontiers in Physiology 11: 596314. Assay: NADP and NADPH in human cells.

Zhang, C., et al. (2020). Metabolic engineering of an auto-regulated Corynebacterium glutamicum chassis for biosynthesis of 5-aminolevulinic acid. Bioresource Technology 318: 124064. Assay: NADP and NADPH in C. glutamicum.

Liu, Y., et al. (2020). Distinct roles of alternative oxidase pathway during the greening process of etiolated algae. Science China. Life Sciences. Assay: NADP and NADPH in algal cells.

Gong, Q.-Y., et al. (2020). Metabolic modulation of redox state confounds fish survival against Vibrio alginolyticus infection. Microbial Biotechnology 13(3): 796-812. Assay: NADP and NADPH in zebrafish cells.

Tomas-Gamisans, M., et al. (2020). Redox engineering by ectopic overexpression of NADH kinase in recombinant Pichia pastoris (Komagataella phaffii): Impact on cell physiology and recombinant production of secreted proteins. Applied and Environmental Microbiology 86(6). Assay: NADP and NADPH in yeast cells.

Hu, Li, et al (2019). Melatonin decreases M1 polarization via attenuating mitochondrial oxidative damage depending on UCP2 pathway in prorenin-treated microglia. PloS one 14.2: e0212138. Assay: NADP/NADPH in rat microglial cells.

You, Sung-Hwan, et al (2019). Rapid and sensitive detection of NADPH via mBFP-mediated enhancement of its fluorescence. PloS one 14.2: e0212061. Assay: NADP/NADPH in bacteria cells.

Jiang, L-B., et al (2018). TIGAR mediates the inhibitory role of hypoxia on ROS production and apoptosis in rat nucleus pulposus cells. Osteoarthritis and cartilage 26.1: 138-148. Assay: NADP/NADPH in rat cells.

Khadka, Dipendra, et al (2018). Augmentation of NAD+ levels by enzymatic action of NAD (P) H quinone oxidoreductase 1 attenuates adriamycin-induced cardiac dysfunction in mice. Journal of molecular and cellular cardiology 124: 45-57. Assay: NADP/NADPH in mice tissues.

Kim, Jae-Eung, et al (2018). Rerouting of NADPH synthetic pathways for increased protopanaxadiol production in Saccharomyces cerevisiae. Scientific reports 8.1: 15820. Assay: NADP/NADPH in yeast cells.

Kwak, Min-Kyu, MyungHee Ku, and Sa-Ouk Kang (2018). Inducible NAD (H)-linked methylglyoxal oxidoreductase regulates cellular methylglyoxal and pyruvate through enhanced activities of alcohol dehydrogenase and methylglyoxal-oxidizing enzymes in glutathione-depleted Candida albicans. Biochimica et Biophysica Acta (BBA)-General Subjects 1862.1: 18-39. Assay: NADP/NADPH in C. albicans (fungi) cells.

Upadhyay, Rashmi, et al (2018). Host Directed Therapy for Chronic Tuberculosis via Intrapulmonary Delivery of Aerosolized Peptide Inhibitors Targeting the IL-10-STAT3 Pathway. Scientific reports 8.1: 16610. Assay: NADP/NADPH in mice tissues.

Wang, Weixuan, et al (2018). ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD+-Mediated Epithelial-Mesenchymal Transition. Cell chemical biology 25.3: 255-261. Assay: NADP/NADPH in human cells.

Fu, Ling, et al (2017). Systematic and quantitative assessment of hydrogen peroxide reactivity with cysteines across human proteomes. Molecular & Cellular Proteomics 16.10: 1815-1828. Assay: NADP/NADPH in human cells.

Ou, Weijun, et al (2017). Low-density lipoprotein docosahexaenoic acid nanoparticles induce ferroptotic cell death in hepatocellular carcinoma. Free Radical Biology and Medicine 112: 597-607. Assay: NADP/NADPH in human cells.

Shin, YoungHo, et al (2017). Cytochrome c peroxidase regulates intracellular reactive oxygen species and methylglyoxal via enzyme activities of erythroascorbate peroxidase and glutathione-related enzymes in Candida albicans. The international journal of biochemistry & cell biology 92: 183-201. Assay: NADP/NADPH in C. albicans (fungi) cells.

Hatori, Yuta, et al (2016). Neuronal differentiation is associated with a redox-regulated increase of copper flow to the secretory pathway. Nature communications 7: 10640. Assay: NADP/NADPH in human cells.

Zhou, Jun-Hao, et al (2016). TIGAR contributes to ischemic tolerance induced by cerebral preconditioning through scavenging of reactive oxygen species and inhibition of apoptosis. Scientific reports 6: 27096. Assay: NADP/NADPH in mice cells.

Csak, T., et al. (2011). Deficiency in myeloid differentiation factor-2 and toll-like receptor 4 expression attenuates nonalcoholic steatohepatitis and fibrosis in mice. Am J Physiol Gastrointest Liver Physiol 300(3):G433-41. Assay: NADP/NADPH in mouse.

Lee, W.H., et al. (2011). Enhanced production of GDP-L: -fucose by overexpression of NADPH regenerator in recombinant Escherichia coli. Appl Microbiol Biotechnol 91(4):967-76. Assay: NADP/NADPH in Escherichia coli bacteria.

Matsunami, T., et al. (2011). Enhancement of reactive oxygen species and induction of apoptosis in streptozotocin-induced diabetic rats under hyperbaric oxygen exposure. Int J Clin Exp Pathol 4(3):255-66. Assay: NADP/NADPH in rat pancreas.

Siedler, S., et al. (2011). Increased NADPH availability in Escherichia coli: improvement of the product per glucose ratio in reductive whole-cell biotransformation. Appl Microbiol Biotechnol. 92(5):929-37. Assay: NADP/NADPH in Escherichia coli bacteria.

Tang, G.J., et al. (2011). Novel role of AMP-activated protein kinase signaling in cigarette smoke induction of IL-8 in human lung epithelial cells and lung inflammation in mice. Free Radic Biol Med 50(11):1492-502. Assay: NADP/NADPH in human lung epithelial cells.

Brown, S.M., et al. (2010). Isocitrate dehydrogenase is important for nitrosative stress resistance in Cryptococcus neoformans, but oxidative stress resistance is not dependent on glucose-6-phosphate dehydrogenase. Eukaryot Cell 9(6):971-80. Assay: NADP/NADPH in Cryptococcus neoformans yeast.

Cao, X., et al. (2010). The effects of quercetin in cultured human RPE cells under oxidative stress and in Ccl2/Cx3cr1 double deficient mice. Exp Eye Res 91(1):15-25. Assay: NADP/NADPH in mouse serum.

Du, J., et al. (2010). Mechanisms of ascorbate-induced cytotoxicity in pancreatic cancer. Clin Cancer Res 16(2):509-20. Assay: NADP/NADPH in human panceatic cancer cells.

Lee, H.C., et al. (2010). High NADPH/NADP+ ratio improves thymidine production by a metabolically engineered Escherichia coli strain. J Biotechnol 149(1-2):24-32. Assay: NADP/NADPH in Escherichia coli bacteria.

Lui, V.W., et al. (2010). An RNA-directed nucleoside anti-metabolite, 1-(3-C-ethynyl-beta-d-ribo-pentofuranosyl)cytosine (ECyd), elicits antitumor effect via TP53-induced Glycolysis and Apoptosis Regulator (TIGAR) downregulation. Biochem Pharmacol 79(12):1772-80. Assay: NADP/NADPH in human cancer cell lines.

Osada-Oka, M., et al. (2010). Glucose is necessary for stabilization of hypoxia-inducible factor-1alpha under hypoxia: contribution of the pentose phosphate pathway to this stabilization. FEBS Lett 584(14):3073-9. Assay: NADP/NADPH in human cells.

Summermatter, S., et al. (2010). Peroxisome proliferator-activated receptor {gamma} coactivator 1{alpha} (PGC-1{alpha}) promotes skeletal muscle lipid refueling in vivo by activating de novo lipogenesis and the pentose phosphate pathway. J Biol Chem 285(43):32793-800. Assay: NADP/NADPH in mice muscle tissue.

Ding, X., et al. (2009). Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci 50(10):4957-66. Assay: NADP/NADPH in mouse serum.

Guo, S., et al. (2009). Specific inhibition of hypoxia inducible factor 1 exaggerates cell injury induced by in vitro ischemia through deteriorating cellular redox environment. J Neurochem 108(5):1309-21. Assay: NADP/NADPH in human neuroblastoma cells.

Korn, A., et al. (2009). Ferredoxin:NADP+ oxidoreductase association with phycocyanin modulates its properties. J Biol Chem 284(46):31789-97. Assay: NADP/NADPH in Synechocystis sp. cyanobacteria.

Lee, H.C., et al. (2009). Thymidine production by overexpressing NAD+ kinase in an Escherichia coli recombinant strain. Biotechnol Lett 31(12):1929-36. Assay: NADP/NADPH in Escherichia coli bacteria.

Liu, M., et al. (2009). Cardiac Na+ current regulation by pyridine nucleotides. Circ Res 105(8):737-45. Assay: NADP/NADPH in human HEK cells.

Tseng, H.C., et al. (2009). Metabolic engineering of Escherichia coli for enhanced production of (R)- and (S)-3-hydroxybutyrate. Appl Environ Microbiol 75(10):3137-45. Assay: NADP/NADPH in Escherichia coli bacteria.

Domain, F., et al. (2007). Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR. Mol Microbiol 66(2):383-94. Assay: NADP/NADPH in bacteria E.coli.

To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.
Buy Now!!!
EnzyChrom™ NADP/NADPH Assay Kit
Catalog No: ECNP-100
Price: $429     Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment:Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C
NADP/NADPH Assay Kit (ECNP-100)

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