EnzyChrom™ Malate Dehydrogenase Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 65 U/L for 20 min reaction at 37°C.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD565nm

Samples
Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media).

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
0.5 U/L

Shelf Life
6 months

More Details
Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer’s disease. BioAssay Systems’ non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
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1. Fan, C et al (2015). Rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acids. Nucl. Acids Res. 43(22):e156. Assay: Cells in E. Coli. (Pubmed).

2. Trivedi, AK et al (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comp. Biochem. Physiol. A 184: 34-40. Assay: Plasma In Avians. (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Malate Dehydrogenase Assay Kit
Catalog No: EMDH-100
Price: $339    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

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Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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