EnzyChrom™ Malate Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism.

Key Features
Fast and sensitive. Use of 20 μL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
OD565nm

Samples
Malate in food, juice, beverage and other agricultural products.

Species
All

Procedure
15 min

Size
100 tests

Detection Limit
0.02 mM

Shelf Life
6 months

More Details
L-malate , or L-Malic acid, is a dicarboxylic acid that is made by all living organisms and plays an important role in the Calvin and Krebs Cycle. It is a source of CO2 for the Calvin cycle in plants and is also an intermediate that forms from fumarate in the Krebs Cycle. Malate is frequently used in food and beverage industries as an additive in products such as wine, beer, candies, etc. BioAssay Systems L-malate assay kit is based on malate dehydrogenase catalyzed oxidation of malate in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm is proportional to the malate concentration in the sample.
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EnzyChrom™ Malate Assay Kit
Catalog No: EMAL-100
Price: $329    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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