QuantiChrom™ Magnesium Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.

Key Features
Sensitive and accurate. Use as little as 5 μL sample. Linear detection range 0.1 mg/dL (41 μM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.

Low interference in biological samples. Assays can be directly performed in serum and urine samples.

Method
OD500nm

Samples
Biological, food and beverage

Species
All

Procedure
10 min

Size
250 tests

Detection Limit
0.1 mg/dL (41 μM)

Shelf Life
12 months

More Details
Magnesium (Mg) is one of the most abundant and essential minerals in mammals.Magnesium is involved in more than 300 biochemical reactions in the body and plays important roles in muscle and nerve functions, heart rhythm, immune system and bone formation. Magnesium deficiency may lead to nausea, fatigue, muscle contractions, hypocalcemia and hypokalemia. Simple, direct and automation-ready procedures for measuring magnesium concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems’ magnesium assay kit is designed to measure magnesium directly in biological samples without any pretreatment. A calmagite dye in the kit forms a colored complex specifically with magnesium. The intensity of the color, measured at 500 nm, is directly proportional to the magnesium concentration in the sample. The optimized formulation minimizes interference by potential substances.

1. What are typical optical density readings of deionized water and the magnesium standard in this assay?



The OD reading will vary with the experimental conditions and the OD reader used. In our laboratory, the OD(500 nm) for distilled water is typically around 0.300, and after the addition of EDTA basically unchanged, for instance 0.296. For the magnesium standard, the values are typically around 0.450 and 0.295, respectively.

2. What is the variability across replicated well samples?



The intra-assay variability is typically below 5%.

3. Do you recommend me to dilute my samples for the Magnesium Assay Kit?



Whether you need to dilute your sample depends on the nature of the sample and the expected concentration of Mg. Normal Mg levels in blood are 1.7-2.3 mg/dL and fall within the linear detection range of 0.1 to 3 mg/dL - dilution is thus not necessary. On the other hand for urine samples, normal values are typically higher, around 7.2 -10.8 mg/dL, and a dilution of the sample is necessary.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Stippler, M et al (2007) Serum and Cerebrospinal Fluid Magnesium in Severe Traumatic Brain Injury Outcome. J Neurotrauma. 24(8): 1347-1354. Assay: Magnesium in Human cerbrospinal fluid (Pubmed).

2. Elizondo MR et al (2010). trpm7 regulation of in vivo cation homeostasis and kidney function involves stanniocalcin 1 and fgf23. Endocrinology 151(12):5700-9. Assay: Magnesium in Zebrafish blood (Pubmed).

3. Kim TW et al (2006) Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system. J Biotechnol 124(2):373-80. Assay: Magnesium in cell free system protein (Pubmed).

4. Pachikian BD et al (2010). Changes in intestinal bifidobacteria levels are associated with the inflammatory response in magnesium-deficient mice. J Nutr. 140(3):509-14. Assay: Magnesium in Mouse serum (Pubmed).

To find more recent publications, please click here.
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QuantiChrom™ Magnesium Assay Kit
Catalog No: DIMG-250
Price: $299    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4°C



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