SuperLight™ Luciferase Reporter Gene Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.

Key Features
High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.

Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.

Fast and convenient: homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.

Robust and amenable to HTS: Z’ factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method
Luminescence

Samples
Cells etc

Species
All

Procedure
2 min

Size
200 tests

Detection Limit
2 fg luciferase

Shelf Life
12 months

More Details
The SuperLight™ Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer. This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the SuperLight™ luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kit include gene regulation studies and high-throughput screening of gene modulators.

1. Can we determine ATP concentration in E. coli cells?



Yes. Ishida A (2002, Anal. Biochem 305, 236-241) described using equal volumes of 10% TCA and E. coli cell culture. Let stand 3 min at room temp. Mix 1 vol of the cell extract with 19 vol of 25 mM Hepes, 17 mM NaOH, and adjust pH to 7.8.before assaying.

2. How to adapt your protocol for adherent cells when working in a 24-well format?



Lyse the cells in the 24 well plate, then add 50 µL cell lysate to 50 µL SuperLight Reagent (10 µL + 10 µL would work too). Alternatively, cells could be harvested with Trypsin/EDTA, resuspend cells in the same volume (200-500 µL of medium). Do the 50 μL + 50 μL assay.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Gentry M et al (2007). Role of Primary Human Alveolar Epithelial Cells in Host Defense against Francisella tularensis Infection. Infection and Immunity 75(8): 3969-3978. Assay: Luciferase Reporter Gene in Human cell line (Pubmed).

2. Labuzek K et al (2010).Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures. Naunyn Schmiedebergs Arch Pharmacol. 381(1):41-57. Assay: Luciferase Reporter Gene in Human cell line (Pubmed).

3. Łabuzek K et al (2010). Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures. Pharmacol Rep. 62(5):827-48. Assay: Luciferase Reporter Gene in Human cell line (Pubmed).

4. Mayer-Wagner S et al (2011). Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells. Bioelectromagnetics. 32(4):283-90. Assay: Luciferase Reporter Gene in monkey vero cells (Pubmed).

5. Zhao L, Haslam DB (2005). A quantitative and highly sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis. J Med Microbiol 54:1023–1030. Assay: Luciferase Reporter Gene in Human cell line (Pubmed).

6. Saenz JB et al (2007). Identification and Characterization of Small Molecules That Inhibit Intracellular Toxin Transport. Infection and Immunity 75(9): 4552–4561. Assay: Luciferase Reporter Gene in monkey cell line (Pubmed).

7. Roupelieva M et al (2010). Kaposi’s sarcoma-associated herpesvirus Lana-1 is a major activator of the serum response element and mitogen-activated protein kinase pathways via interactions with the Mediator complex. J Gen Virol. 91(5):1138-49. Assay: Luciferase Reporter Gene in Mouse cells (Pubmed).

8. McGannon CM et al (2010). Different classes of antibiotics differentially influence shiga toxin production. Antimicrob Agents Chemother. 54(9):3790-8. Assay: Luciferase Reporter Gene in monkey cell line (Pubmed).

9. Kulkarni AA et al (2010). Glycan encapsulated gold nanoparticles selectively inhibit shiga toxins 1 and 2. Bioconjug Chem. 21(8):1486-93. Assay: Luciferase Reporter Gene in monkey cell line (Pubmed).

10. Zaliauskiene L et al (2010). Efficient gene transfection using novel cationic polymers poly(hydroxyalkylene imines). Bioconjug Chem. 21(9):1602-11. Assay: Luciferase Reporter Gene in animal cell line (Pubmed).

11. Fuller CA et al (2011). Shiga toxin subtypes display dramatic differences in potency. Infect Immun. 79(3):1329-37. Assay: Luciferase Reporter Gene in NA cell line (Pubmed).

12. Cohen ME et al (2011). Antibody against extracellular vaccinia virus (EV) protects mice through complement and Fc receptors. PLoS One. 6(6):e20597. Assay: Luciferase Reporter Gene in mice cells (Pubmed).

13. Tannous BA et al (2009). Secreted Luciferase For Ex Vivo Monitoring Of In Vivo Precesses. US2009235370. Assay: Luciferase Reporter Gene in NA NA (Pubmed).

14. Tannous BA et al (2011) Gaussia Luciferase Variant For High-Throughput Screening. WO2011002924. Assay: Luciferase Reporter Gene in NA NA (Pubmed).

To find more recent publications, please click here. To find more recent publications, please click here.
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SuperLight™ Luciferase Reporter Gene Assay Kit
Catalog No: SLLU-200
Price: $119    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C



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