SuperLight™ Luciferase Reporter Gene Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.

Key Features
High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.

Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.

Fast and convenient: homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.

Robust and amenable to HTS: Z factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method
Luminescence

Samples
Cells etc

Species
All

Procedure
2 min

Size
1000 tests

Detection Limit
2 fg luciferase

Shelf Life
12 months

More Details
The SuperLight™ Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer. This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the SuperLight™ luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kit include gene regulation studies and high-throughput screening of gene modulators.

1. Can we determine ATP concentration in E. coli cells?



Yes. Ishida A (2002, Anal. Biochem 305, 236-241) described using equal volumes of 10% TCA and E. coli cell culture. Let stand 3 min at room temp. Mix 1 vol of the cell extract with 19 vol of 25 mM Hepes, 17 mM NaOH, and adjust pH to 7.8.before assaying.

2. How to adapt your protocol for adherent cells when working in a 24-well format?



Lyse the cells in the 24 well plate, then add 50 µL cell lysate to 50 µL SuperLight Reagent (10 µL + 10 µL would work too). Alternatively, cells could be harvested with Trypsin/EDTA, resuspend cells in the same volume (200-500 µL of medium). Do the 50 µL + 50 μL assay.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Cohen ME, et al (2011). Antibody against extracellular vaccinia virus (EV) protects mice through complement and Fc receptors. PLoS One. 6(6):e20597. Assay: Reporter gene in monkey cell line.

Fuller CA, et al (2011). Shiga toxin subtypes display dramatic differences in potency. Infect Immun. 79(3):1329-37. Assay: Reporter gene in monkey cell line.

Mayer-Wagner S et al (2011). Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells. Bioelectromagnetics. 32(4):283-90. Assay: Luciferase reporter gene in monkey vero cells.

Tannous BA et al (2011). Gaussia Luciferase Variant For High-Throughput Screening. WO2011002924. Assay: Reporter gene in mice.

Kulkarni AA, et al (2010). Glycan encapsulated gold nanoparticles selectively inhibit shiga toxins 1 and 2. Bioconjug Chem. 21(8):1486-93. Assay: Reporter gene in monkey cell line.

Labuzek K et al (2010). Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures. Naunyn Schmiedebergs Arch Pharmacol. 381(1):41-57. Assay: Luciferase reporter gene in human cell line.

Labuzek K et al (2010). Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures. Pharmacol Rep. 62(5):827-48. Assay: Luciferase reporter gene in human cell line.

McGannon CM, et al (2010). Different classes of antibiotics differentially influence shiga toxin production. Antimicrob Agents Chemother. 54(9):3790-8. Assay: Reporter gene in human cell line.

Roupelieva M, et al (2010). Kaposi's sarcoma-associated herpesvirus Lana-1 is a major activator of the serum response element and mitogen-activated protein kinase pathways via interactions with the Mediator complex. J Gen Virol. 91(5):1138-49. Assay: Reporter gene in human cell line.

Zaliauskiene L, et al (2010). Efficient gene transfection using novel cationic polymers poly(hydroxyalkylene imines). Bioconjug Chem. 21(9):1602-11. Assay: Reporter gene in mice.

Tannous, BA. et al (2009). Secreted Luciferase For Ex Vivo Monitoring Of In Vivo Precesses. US2009235370. Assay: Reporter gene in mice.

Gentry, M. et al. (2007). Role of Primary human Alveolar Epithelial Cells in Host Defense against Francisella tularensis Infection. Infection and Immunity 75(8): 3969-3978. Assay: Reporter gene in human cell line.

Michael, K. et al. (2007). Microplate Orbital Mixing Improves High-Throughput Cell-Based Reporter Assay Readout. J Biomol Screen 12(1):140-144. Assay: Reporter gene in mouse 3T3 cells.

Saenz JB,et al (2007). Identification and Characterization of Small Molecules That Inhibit Intracellular Toxin Transport. Infection and Immunity 75(9): 4552-4561. Assay: Reporter gene in monkey cell line.

Zhao, L. and Haslam, D.B. (2005). A quantitative and highly sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis. J Med Microbiol 54:1023-1030. Assay: Reporter gene in human cell line.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
SuperLight™ Luciferase Reporter Gene Assay Kit
Catalog No: SLLU-01K
Price: $419    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers