EnzyChrom™ Lactose Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

Key Features
Use as little as 20 μL samples. Linear detection range in 96-well plate: 17 to 2000 μM lactose for colorimetric assays and 6 to 100 μM for fluorimetric assays.

Method
OD570nm, or FL530/585nm

Samples
Biological (milk), food and agriculture etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
6 μM

Shelf Life
12 months

More Details
Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems’ assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.

1. I would like to know if I can use the optical density at 540nm to read my samples.



The signal at 540nm is only about 2/3 of the signal at 570 nm, but you still can use it. However, you will lose sensitivity.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Kwon, DH et al (2012). Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. Journal of Nutritional Biochemistry 23(7): 791-9. Assay: Milk in Rats (Pubmed).

2. Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44. Assay: Liposomes (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Lactose Assay Kit
Catalog No: ELAC-100
Price: $319    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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