EnzyFluo™ D-Lactate Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features
Sensitive and accurate. Detection limit of 1 μM and linearity up to 50 μM D-lactate in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
FL530/585 nm

Samples
Serum, plasma, cell culture media etc

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
1 μM

Shelf Life
6 months

More Details
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.
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EnzyFluo™ D-Lactate Assay Kit
Catalog No: EFDLC-100
Price: $329    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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