EnzyChrom™ D-Lactate Assay Kit
Datasheet PDF  MSDS/Bulk Order

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features
Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

OD565 nm

Serum, plasma, cell culture media etc


20 min

100 tests

Detection Limit
0.05 mM

Shelf Life
6 months

More Details
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
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1. Kurundkar, AR et al (2010). Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res 68(2):128-33. Assay: D-Lactate in Pig Plasma (Pubmed).

2. Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in Human Serum (Pubmed).

3. Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/ NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: Pulmonary Artery Andothelial Cells in Humans (Pubmed).

4. Raycol, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: pancreatic tumor cells in Rats and Mice (Pubmed).

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EnzyChrom™ D-Lactate Assay Kit
Catalog No: EDLC-100
Price: $329    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C

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