EnzyChrom™ D-Lactate Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features
Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
OD565 nm

Samples
Serum, plasma, cell culture media etc

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
0.05 mM

Shelf Life
6 months

More Details
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
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1. Kurundkar, AR et al (2010). Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res 68(2):128-33. Assay: D-Lactate in Pig Plasma (Pubmed).

2. Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in Human Serum (Pubmed).

3. Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/ NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: Pulmonary Artery Andothelial Cells in Humans (Pubmed).

4. Raycol, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: pancreatic tumor cells in Rats and Mice (Pubmed).

To find more recent publications, please click here. To find more recent publications, please click here.
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EnzyChrom™ D-Lactate Assay Kit
Catalog No: EDLC-100
Price: $329    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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