EnzyChrom™ Ketone Body Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism.

Key Features
Sensitive and accurate. Uses 10 μL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature.

High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.

Method
OD340nm

Samples
Serum, plasma, urine etc

Species
All

Procedure
30 min

Size
200 tests

Detection Limit
0.12 mM

Shelf Life
6 months

More Details
Ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) are produced in the liver mainly from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypo-glycaemia, growth hormone deficiency, alcohol or salicylate intoxication and inborn errors of metabolism. Simple, direct and automation-ready procedures for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH) are very desirable. BioAssay Systems EnzyChrom™ ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations,

1. Can serum and plasma samples be stored frozen?



β-hydroxybutyrate is stable and can be detected in samples that had been stored frozen for more than a month. Acetoacetate (AcAc), on the other hand, is an unstable compound which becomes rapidly decarboxylated. We recommend to use fresh serum or freeze serum samples at -80°C for up to one month.

2. I seem to be getting very low levels of BOH and AcAc in freshly drawn blood. Perhaps this is because they are breaking down within the hour it takes me to process the plasma out.



In healthy individuals, normal blood values for ketone bodies are actually very low or not detectable. Elevated blood levels of ketone bodies (ketosis) can be caused for example by diabetes, alcohol intoxication, starvation, or physical exercise. You can check for the stability of your analyte by setting aside an aliquot of your sample and add the standard as a positive control.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Klusonova P et al (2011) Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats--effect of hypertriglyceridemia and gender. Steroids 76(12):1252-9. Assay: Ketone Body in Rat Hepatocyte culture (Pubmed).

2. Sharma NS et al (2011). Metabolic profiling based quantitative evaluation of hepatocellular metabolism in presence of adipocyte derived extracellular matrix. PLoS One 6(5):e20137. Assay: Ketone Body in Human Serum (Pubmed).

To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please inquire via email or call 1-510-782-9988 x 2.
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EnzyChrom™ Ketone Body Assay Kit
Catalog No: EKBD-100
Price: $389    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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