EnzyChrom™ Isocitrate Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 20 to 5000 μM for 10 min reaction.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD565nm

Samples
Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
20 μM

Shelf Life
6 months

More Details
ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.
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EnzyChrom™ Isocitrate Assay Kit
Catalog No: ECIC-100
Price: $309    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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