EnzyChrom™ Intestinal Permeability Assay Kit
Protocol ❘ SDS
BioAssay Systems is going green. Printed protocols and MSDSs will no longer be included with kits. Starting January 1, 2018, protocols and MSDSs will only be available online.
Application
• Determination of intestinal permeability (leaky gut syndrome) through measuring lactulose/mannitol ratio in urine samples.
Key Features
• Simple and convenient. Both assays require addition of single working reagent and can be completed within 60 minutes. Both assays are performed at room temperature. No 37°C heater is needed.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
• OD565nm
Samples
• Urine
Species
• Human
Procedure
• 60 and 30 min
Size
• 100 tests
Detection Limit
• D-Mannitol: 7 μM; Lactulose: 3 μM
Shelf Life
• 6 months
More Details
• INTESTINAL PERMEABILITY is a phenomenon of the gut wall in which leakage of molecules and ions below 0.4 nm occurs from the gut lumen into blood circulation. This paracellular leakage occurs through tight junctions between epithelial cells. Elevated paracellular leakage has been implicated in many disorders including type 1 and type 2 diabetes, obesity, inflammatory bowel disease, celiac disease, Parkinson’s disease and cancer.
BioAssay Systems intestinal permeability assay kit is based on measuring the ratio of the absorption of two non-metabolized sugars to through the intestines. Test subjects drink a prescribed amount of lactulose and mannitol and the % absorption of these sugars is determined by the amount of excreted lactulose and mannitol measured during the first 6 hours after ingestion. The degree of intestinal permeability is reflected by the ratio of the % absorption of lactulose to % absorption of mannitol. An increase in this ratio indicates increased intestinal permeability since lactulose is only absorbed though intercellular spaces. Lactulose and mannitol are measured in separate assays using the included EnzyChrom™ Lactulose Assay Kit and Mannitol Assay Kit, respectively.
BioAssay Systems is going green. Printed protocols and MSDSs will no longer be included with kits. Starting January 1, 2018, protocols and MSDSs will only be available online.
Application
• Determination of intestinal permeability (leaky gut syndrome) through measuring lactulose/mannitol ratio in urine samples.
Key Features
• Simple and convenient. Both assays require addition of single working reagent and can be completed within 60 minutes. Both assays are performed at room temperature. No 37°C heater is needed.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
• OD565nm
Samples
• Urine
Species
• Human
Procedure
• 60 and 30 min
Size
• 100 tests
Detection Limit
• D-Mannitol: 7 μM; Lactulose: 3 μM
Shelf Life
• 6 months
More Details
• INTESTINAL PERMEABILITY is a phenomenon of the gut wall in which leakage of molecules and ions below 0.4 nm occurs from the gut lumen into blood circulation. This paracellular leakage occurs through tight junctions between epithelial cells. Elevated paracellular leakage has been implicated in many disorders including type 1 and type 2 diabetes, obesity, inflammatory bowel disease, celiac disease, Parkinson’s disease and cancer.
BioAssay Systems intestinal permeability assay kit is based on measuring the ratio of the absorption of two non-metabolized sugars to through the intestines. Test subjects drink a prescribed amount of lactulose and mannitol and the % absorption of these sugars is determined by the amount of excreted lactulose and mannitol measured during the first 6 hours after ingestion. The degree of intestinal permeability is reflected by the ratio of the % absorption of lactulose to % absorption of mannitol. An increase in this ratio indicates increased intestinal permeability since lactulose is only absorbed though intercellular spaces. Lactulose and mannitol are measured in separate assays using the included EnzyChrom™ Lactulose Assay Kit and Mannitol Assay Kit, respectively.
Please refer to FAQs for EnzyChrom™ D-Mannitol Assay Kit (cat#: EMNT-100) and EnzyChrom™ Lactulose Assay Kit (cat#: ELTL-100)
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
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Expert Technical Support
Technical support provided by the very scientists that develop the assays.
Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market
Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.
Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.
Technical support provided by the very scientists that develop the assays.
Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market
Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.
Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.
BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers
