EnzyChrom™ HDL and LDL/VLDL Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

Key Features
Sensitive and accurate. Requires only 20 μL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.

Convenient. Room temperature assay. No 37°C heater is needed.

Method
OD340nm

Samples
Serum

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
5 mg/dL

Shelf Life
6 months

More Details
Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems’ HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

1. Would you please confirm me cat# EHDL-100 can be used for Rat or not?



Yes, the assay is not species-specific and works with rat serum samples.

2. Could they be used with lung tissue homogenates?



Yes, but the EnzyChrom™ HDL and LDL/VLDL assay kits were developed for serum samples to detect the various transport forms of cholesterol. For tissue samples I would rather recommend using our cholesterol assay kits ECCH-100/E2CH-100.

3. We would like to know if, during the procedure and before the OD measurement, the reagent added to the serum inactivates hepathitis C virus.



No. The EHDL-100 assay reagents will not inactivate hepatitis C virus, or any other virus.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-84. Assay: HDL/LDL in mice plasma (Pubmed).

2. Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDL/LDL in pig serum (Pubmed).

3. Labib, HM et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: HDL/LDL in Mouse Blood (Pubmed).

4. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in Human Plasma (Pubmed).

5. Shanmugasundaram R & Selvaraj RK (2011) Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in Mouse blood (Pubmed).

6. Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL/LDL in Rat Serum (Pubmed).

7. Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in Bird serum (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ HDL and LDL/VLDL Assay Kit
Catalog No: EHDL-100
Price: $379    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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