EnzyChrom™ HDL and LDL/VLDL Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

Key Features
Sensitive and accurate. Requires only 20 μL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.

Convenient. Room temperature assay. No 37°C heater is needed.

Method
OD340nm

Samples
Serum

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
5 mg/dL

Shelf Life
6 months

More Details
Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

1. Would you please confirm me cat# EHDL-100 can be used for Rat or not?



Yes, the assay is not species-specific and works with rat serum samples.

2. Could they be used with lung tissue homogenates?



Yes, but the EnzyChrom™ HDL and LDL/VLDL assay kits were developed for serum samples to detect the various transport forms of cholesterol. For tissue samples I would rather recommend using our cholesterol assay kits ECCH-100/E2CH-100.

3. We would like to know if, during the procedure and before the OD measurement, the reagent added to the serum inactivates hepathitis C virus.



No. The EHDL-100 assay reagents will not inactivate hepatitis C virus, or any other virus.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Jager, M., Hubert, A., Gogiraju, R., Bochenek, M. L., Munzel, T., & Schafer, K. (2019). Inducible knockdown of endothelial protein tyrosine phosphatase-1B promotes neointima formation in obese mice by enhancing endothelial senescence. Antioxidants & redox signaling, 30(7), 927-944. Assay: HDL and LDL/VLDL in mice serum.

Sharma, V., Smolin, J., Nayak, J., Ayala, J. E., Scott, D. A., Peterson, S. N., & Freeze, H. H. (2018). Mannose Alters Gut Microbiome, Prevents Diet-Induced Obesity, and Improves Host Metabolism. Cell reports, 24(12), 3087-3098. Assay: HDL and LDL/VLDL in mice serum.

Bergt, S., Grub, A., Wagner, S., Engelke, H., Noldge-Schomburg, G., Vollmar, B. & Wagner, N. M. (2017). Pravastatin but not simvastatin improves survival and neurofunctional outcome after cardiac arrest and cardiopulmonary resuscitation. JACC: Basic to Translational Science, 2(2), 149-159. Assay: HDL and LDL/VLDL in mice plasma.

Shon, S. M., Jang, H. J., Schellingerhout, D., Kim, J. Y., Ryu, W. S., Lee, S. K. & Je, K. H. (2017). Cytokine Response to Diet and Exercise Affects Atheromatous Matrix Metalloproteinase-2/9 Activity in Mice. Circulation Journal 81(10):1528-1536. Assay: HDL and LDL/VLDL in mice serum.

Stanton, Catherine, et al (2017). Exopolysaccharide-producing bacteria, and uses thereof for protecting heart health. U.S. Patent No. 9,808,493 Assay: HDL and LDL/VLDL in mice serum.

Frej, C., Linder, A., Happonen, K. E., Taylor, F. B., Lupu, F., & Dahlback, B. (2016). Sphingosine 1-phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons. Journal of cellular and molecular medicine, 20(6), 1170-1181. Assay: HDL and LDL/VLDL in baboon plasma.

Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-84. Assay: HDLandLDL/VLDL in mice plasma.

Shanmugasundaram R & Selvaraj RK (2011). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in mouse blood.

Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDLandLDL/VLDL in rat serum.

Shon, S-M. et al. (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL and LDL/VLDL in mouse blood.

Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDLandLDL/VLDL in pig serum.

Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in bird serum.

Ponda MP, et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: HDLandLDL/VLDL in mouse Blood.

Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in human plasma.

Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: HDLandLDL/VLDL in bird serum.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyChrom™ HDL and LDL/VLDL Assay Kit
Catalog No: EHDL-100
Price: $399    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers