EnzyChrom™ AF HDL and LDL/VLDL Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

Key Features
Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.

Convenient. Room temperature assay. No 37°C heater is needed.

Method
OD570nm, or FL530/585nm

Samples
Serum

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
OD, FL: 1, 0.2 mg/dL

Shelf Life
6 months

More Details
CHOLESTEROL concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems’ HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to total cholesterol concentration in the sample.

1. I want to ask about the differences between "EnzyChrom™ AF HDL and LDL/VLDL Assay Kit and EnzyChrom™ HDL and LDL/VLDL Assay Kit. Since I want to work on mice.



Both kits will work with mouse serum and plasma samples, but I would recommend using serum, because we have not validated the use of plasma. The EHDL-100 kit has a linear detection range of 5 -300 mg/dL. It requires a microplate reader with a 340nm filter. The E2HL-100 kit is more sensitive with a linear detection range of 1 -100 mg/dL using the colorimetric readout (requires a 570 nm filter), or 0.2 -10 mg/dL with the fluorimetric readout (requires a fluorescence microplate reader).

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Tam J et al (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120(8):2953-66. Assay: HDL/LDL in Mouse Serum (Pubmed).

2. Wan W et al (2011). Genetic deletion of chemokine receptor Ccr6 decreases atherogenesis in ApoE-deficient mice. Circ Res. 109(4):374-381. Assay: HDL/LDL in Mouse Serum (Pubmed).

3. Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-484. Assay: HDL/LDL in Mouse Plasma (Pubmed).

4. Tucci, S et al (2010). Medium-chain triglycerides impair lipid metabolism and induce hepatic steatosis in very long-chain acyl-CoA dehydrogenase (VLCAD)-deficient mice. Mol Genet Metab 101(1): 40-47 . Assay: HDL/LDL in Mouse blood (Pubmed).

5. Nagajyothi F et at (2011). Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion. PLoS Negl Trop Dis. 1;5(2):e953. Assay: HDL/LDL in Mouse serum (Pubmed).

6. Uddin, MJ et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genetics 2011, 12:62. Assay: HDL/LDL in Pig Serum (Pubmed).

7. Takakuwa Y et al (2011). AC13, a C-terminal fragment of apolipoprotein A-I, is a candidate biomarker for microscopic polyangiitis. Arthritis Rheum. [Epub ahead of print]. Assay: HDL/LDL in Human blood (Pubmed).

8. Parveen, TD et al (2011). Antihyperlipidemic Activity of the Methanolic extract from the Stems of Tinospora cordifolia on Sprague dawley rats. Der Pharmacia Sinica 2 (1): 104-109. Assay: HDL/LDL in Rat blood (Pubmed).

9. Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: HDL/LDL in Bird Japanese Quail blood (Pubmed).

10. Zhu, FG et al (2010). Use of Inhibitors of Toll-like receptors in the prevention and treatment of hyperchloesterolenia and hyperlipidemia and dreases related thereto. US2010/0098685A1. Assay: HDL/LDL in Mouse blood (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ AF HDL and LDL/VLDL Assay Kit
Catalog No: E2HL-100
Price: $379    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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