EnzyChrom™ Glycerol Assay Kit
Datasheet PDF  MSDS/Bulk Order

For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.

Key Features
Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 10 to 1000 μM (92 μg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 μM for fluorimetric assays.

Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.

Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

OD570nm, or FL530/585nm

Biological, food, beverage etc


20 min

200 tests

Detection Limit
2 μM

Shelf Life
12 months

More Details
Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. BioAssay Systems’ glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Lung, M et al (2009). Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J Biol Chem 284(45):31062-73. Assay: Glycerol in N/A Diacylglycerol (Pubmed).

2. Drew, BG et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res 52(3):572-581. Assay: Glycerol in Human Plasma (Pubmed).

3. Wachtler, B et al (2011). From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS One 6(2):e17046. Assay: Glycerol in Fungi C. albicans (Pubmed).

4. Bahar, B et al. A potential role of IL-6 in the chito-oligosaccharide-mediated inhibition of adipogenesis (2011). Br J Nutr. 106(8):1142-1153. Assay: Glycerol in Mouse 3T3-L1 cells (Pubmed).

5. . Assay: Glycerol in Mouse Plasma (Pubmed).

6. Pifferi, F et al (2011). Mild experimental ketosis increases brain uptake of 11C-acetoacetate and 18F-fluorodeoxyglucose: a dual-tracer PET imaging study in rats. Nutr Neurosci 14(2):51-8. Assay: Glycerol in Rat Plasma (Pubmed).

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EnzyChrom™ Glycerol Assay Kit
Catalog No: EGLY-200
Price: $299    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C

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