QuantiChrom™ Glutathione S-transferase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
Glutathione S-transferase activity determination in biological samples.

Key Features
Fast and sensitive. Linear detection range (20 µL sample): 2 to 80 U/L for 10 min reaction at 25°C.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD340nm

Samples
Biological samples (e.g. cell lysates, tissues, etc.)

Species
All

Procedure
• 10 min

Size
100 tests

Detection Limit
2 U/L

Shelf Life
6 months

More Details
GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctionalenzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.

BioAssay Systems Glutathione S-transferase assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.

1. How do I store the kit?



This kit is shipped at ambient temperature. Upon receiving, please keep the kit at 4°C. Store reconstituted glutathione tube at -20°C and use within 3 weeks.

2. What kind of samples can be used?



This kit can be used in cell, tissue, plasma, and erythrocyte samples, etc.

3. Can I store unused reagents for future use?



Yes, unused reagents can be stored according to the assay protocol. Since glutathione is quite unstable after reconstitution, store unused glutathione at -20°C and avoid repeated freeze/thaw cycles.



For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
No citations for this new product. Please check back later.
You may click here to check if citations are available, but are not listed here yet.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
QuantiChrom™ Glutathione S-transferase Assay Kit
Catalog No: DGST-100
Price: $249    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4°C and -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers