EnzyChrom™ Glutathione Reductase Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of glutathione reductase activity.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD412nm

Samples
Plasma, serum, tissue and culture media etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
0.4 U/L

Shelf Life
6 months

More Details
GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. BioAssay Systems’ non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman’s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.
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1. Bardell, DA et al (2015). Measures of Redox Balance in Horses Undergoing Corrective Surgery Involving Strangulating Lesions of the Small Intestine. Equine Vet J. 47(S48): 6. Assay: Plasma in Horses (Pubmed).

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EnzyChrom™ Glutathione Reductase Kit
Catalog No: ECGR-100
Price: $209    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

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Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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