EnzyChrom™ Glutathione Peroxidase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity.

Key Features
Sensitive and accurate. Use 10 μL sample. Linear detection range 40 to 800 U/L GPX activity.

Method
OD340nm

Samples
Biological

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
40 U/L

Shelf Life
6 months

More Details
Glutathione peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR), Simple, direct and high-throughput assays for GPX activity find wide applications. BioAssay Systems improved assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.
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De Maistre, S., Vallee, N., Gaillard, S., Duchamp, C., & Blatteau, J. E. (2018). Stimulating fermentation by the prolonged acceleration of gut transit protects against decompression sickness. Scientific reports, 8(1), 10128. Assay: Glutahione peroxidase in rat plasma.

Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: Glutahione peroxidase in D. melanogaster tissues.

Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Glutahione peroxidase in rat tissues.

Kook, S. H., Cheon, S. R., Kim, J. H., Choi, K. C., Kim, M. K., & Lee, J. C. (2017). Dietary hydroxycinnamates prevent oxidative damages to liver, spleen, and bone marrow cells in irradiation-exposed mice. Food science and biotechnology, 26(1), 279-285. Assay: Glutahione peroxidase in mice tissues.

Adeyemi, K. D., Ismail, M., Ebrahimi, M., Sabow, A. B., Shittu, R. M., Karim, R., & Sazili, A. Q. (2016). Fatty acids, lipid and protein oxidation, metmyoglobin reducing activity and sensory attributes of biceps femoris muscle in goats fed a canola and palm oil blend. South African Journal of Animal Science, 46(2), 139-151. Assay: Glutahione peroxidase in goat tissues.

Adeyemi, K. D., Sabow, A. B., Aghwan, Z. A., Ebrahimi, M., Samsudin, A. A., Alimon, A. R., & Sazili, A. Q. (2016). Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend. Journal of animal science and technology, 58(1), 6. Assay: Glutahione peroxidase in goat serum.

Adeyemi, K. D., Shittu, R. M., Sabow, A. B., Abubakar, A. A., Karim, R., Karsani, S. A., & Sazili, A. Q. (2016). Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of animal science and technology, 58(1), 23. Assay: Glutahione peroxidase in boer tissues.

Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutahione peroxidase in fish plasma.

Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutathione Peroxidase in fish plasma.

Ahmed, MA et al (2014). Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sciences 110(2): 61-9. Assay: Glutahione peroxidase in rat brain tissue.

Chen, W et al (2014). Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats. PLoS One 9(2):e88076. Assay: Glutahione peroxidase in rat hippocampus tissue.

Effendy, NM and Shuid, AN (2014). Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis rat Model. Nutrients 6(8): 3288-302. Assay: Glutahione peroxidase in rat bone tissue.

Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Toxicological & Environmental Chemistry 96(2): 307-317. Assay: Glutahione peroxidase in rat.

Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione peroxidase in deer blood.

Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione Peroxdiase in Deer Blood.

Calamita G et al (2012). Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol. Biol Cell 104(6):342-51. Assay: Glutahione peroxidase in human blood.

Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Glutathione Peroxidase in human blood.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Glutathione Peroxidase Assay Kit
Catalog No: EGPX-100
Price: $259    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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