EnzyChrom™ GSH/GSSG Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).

Key Features
Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Method
OD412nm

Samples
Whole blood, plasma, serum, urine, tissue and cell extracts

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
10 nM

Shelf Life
6 months

More Details
Glutathione , a tripeptide of glycine, glutamic acid and cysteine, is one of the key antioxidants involved in protecting cells from damages by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems’ GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
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EnzyChrom™ GSH/GSSG Assay Kit
Catalog No: EGTT-100
Price: $249    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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