EnzyChrom™ GSH/GSSG Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).

Key Features
Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Method
OD412nm

Samples
Whole blood, plasma, serum, urine, tissue and cell extracts

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
10 nM

Shelf Life
6 months

More Details
Glutathione , a tripeptide of glycine, glutamic acid and cysteine, is one of the key antioxidants involved in protecting cells from damages by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please inquire via email or call 1-510-782-9988 x 2.
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EnzyChrom™ GSH/GSSG Assay Kit
Catalog No: EGTT-100
Price: $269    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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