EnzyChrom™ GSH/GSSG Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).

Key Features
Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Method
OD412nm

Samples
Whole blood, plasma, serum, urine, tissue and cell extracts

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
10 nM

Shelf Life
6 months

More Details
Glutathione , a tripeptide of glycine, glutamic acid and cysteine, is one of the key antioxidants involved in protecting cells from damages by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
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Lin, T. A., Ke, B. J., Cheng, C. S., Wang, J. J., Wei, B. L., & Lee, C. L. (2019). Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-beta1 Pathway. Nutrients, 11(2), 395. Assay: GSH/GSSG in mice tissues.

Kim, K. H., Kim, E. Y., Lee, S. Y., Ko, J. J., & Lee, K. A. (2018). Oocyte Cytoplasmic Gas6 and Heparan Sulfate (HS) are Required to Establish the Open Chromatin State in Nuclei During Remodeling and Reprogramming. Cellular Physiology and Biochemistry, 45(1), 37-53. Assay: GSH/GSSG in mice cells.

Li, X. X., Wang, Z. J., Zheng, Y., Guan, Y. F., Yang, P. B., Chen, X. & Chien, K. Y. (2018). Nuclear receptor Nur77 facilitates melanoma cell survival under metabolic stress by protecting fatty acid oxidation. Molecular cell, 69(3), 480-492. Assay: GSH/GSSG in mice cells.

Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: GSH/GSSG in D. melanogaster tissues.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: GSH/GSSG in rat tissues and plasma.

Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: GSH/GSSG in human tissues.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2017). Deciphering Molecular Cascades In A Novel Acclimatization Strategy For Rapid Ascent To High Altitude. bioRxiv, 145342. Assay: GSH/GSSG in rat tissues and plasma.

Khatri, R., Krishnan, S., Roy, S., Chattopadhyay, S., Kumar, V., & Mukhopadhyay, A. (2016). Reactive oxygen species limit the ability of bone marrow stromal cells to support hematopoietic reconstitution in aging mice. Stem cells and development 25(12):948-58. Assay: GSH/GSSG in mice stromal cells.

Lee, H. N., et al. (2015). Heme Oxygenase-1 Determines the Differential Response of Breast Cancer and Normal Cells to Piperlongumine. Molecules and cells, 38(4), 327. Assay: GSH/GSSG in piperlongumine cell.

Chatterjee, N., Eom, H. J., & Choi, J. (2014). A systems toxicology approach to the surface functionality control of graphene-cell interactions. Biomaterials, 35(4), 1109-1127. Assay: GSH/GSSG in human HepG2 cells.

Di Maro, G., et al. (2014). Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. Molecular cancer, 13(1), 1. Assay: GSH/GSSG in human tissues.

Muller, S., et al. (2014). Age-dependent effects of UCP2 deficiency on experimental acute pancreatitis in mice. PloS one, 9(4), e94494. Assay: GSH/GSSG in mice serum.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ GSH/GSSG Assay Kit
Catalog No: EGTT-100
Price: $269    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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