QuantiChrom™ Glutathione (GSH) Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of reduced glutathione (GSH) and evaluation of drug effects on glutathione metabolism.

Key Features
Sensitive and accurate. Linear detection range 0.4 - 100 μM in 96-well plate.

Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density.

Low interference. Amino acids and common buffers do not interfere.

Method
OD412nm

Samples
Whole blood, plasma, serum, urine, tissue and cell extracts

Species
All

Procedure
30 min

Size
250 tests

Detection Limit
12 μg/dL (0.4 μM)

Shelf Life
12 months

More Details
Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase. Simple, direct and automation-ready procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ Glutathione Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5’-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. The optimized formulation has a long shelf life and is completely free of interference due to sample turbidity.

1. We would like to know if the QuantiChrom glutathione assay kit ref: DIGT-250 can be used to detect glutathione in wine.



We tested a few wine samples (red and white) with our DIGT-250 kit. Unfortunately, the wine gave too high of a background at 412 nm and the glutathione levels are too low in wine for dilution of the sample to work. Therefore, we cannot recommend using this kit for glutathione determination in wine.

2. The protocol does not mention sample collection methods (i.e. whether it requires special preservatives, stabilizers etc.). What are your recommendations?



We recommend processing serum samples immediately and avoiding prolonged storage. Rapid acidification is required to prevent GSH auto-oxidation, GSH and GSSG degradation, as well as to precipitate proteins that interfere with analysis. Although we have not validated it, we believe the processed samples should be stable at -80°C.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Hassannia, B., Wiernicki, B., Ingold, I., Qu, F., Van Herck, S., Tyurina, Y. Y. & Meul, E. (2018). Nano-targeted induction of dual ferroptotic mechanisms eradicates high-risk neuroblastoma. Journal of Clinical Investigation, 128(8), 3341-3355. Assay: Glutathione in human cells.

Lee, H. J., Han, J. H., Park, Y. K., & Kang, M. H. (2018). Effects of glutathione s-transferase (GST) M1 and T1 polymorphisms on antioxidant vitamins and oxidative stress-related parameters in Korean subclinical hypertensive subjects after kale juice (Brassica oleracea acephala) supplementation. Nutrition research and practice, 12(2), 118-128. Assay: Glutathione in human blood.

Mizrahi, M., Adar, T., Lalazar, G., Nachman, D., El Haj, M., Ya'acov, A. B. & Ilan, Y. (2018). Glycosphingolipids Prevent APAP and HMG-CoA Reductase Inhibitors-mediated Liver Damage: A Novel Method for "Safer Drug" Formulation that Prevents Drug-induced Liver Injury. Journal of clinical and translational hepatology 6(2): 127-134. Assay: Glutathione in mice cells.

Saint-Germain, E., Mignacca, L., Vernier, M., Bobbala, D., Ilangumaran, S., & Ferbeyre, G. (2017). SOCS1 regulates senescence and ferroptosis by modulating the expression of p53 target genes. Aging (Albany NY) 9(10): 2137-2162. Assay: Glutathione in human cells.

Jaikumkao, K., Pongchaidecha, A., Chattipakorn, N., Chatsudthipong, V., Promsan, S., Arjinajarn, P., & Lungkaphin, A. (2016). Atorvastatin improves renal organic anion transporter 3 and renal function in gentamicin-induced nephrotoxicity in rats. Experimental physiology, 101(6), 743-753. Assay: Glutathione in Sprague Dewley rats renal tissue.

Suh, KS et al (2016). Protective effects of honokiol against methylglyoxal-inducedosteoblast damage. Chemico-Biological Interactions. 244:169-177. Assay: Glutathione in plant extract cells.

Barros, MA et al (2015). L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients. Transplantation Proceedings. 47(8):2478-82. Assay: Glutathione in human liver tissue.

Malek, HA et al (2015). The preventive effect of beta3 adrenoceptor stimulation against experimentally induced reflux esophagitis. Acta Physiologica Hungarica. 102(1): 94-104. Assay: Glutathione in rat plasma.

Mesci, P et al (2015). System xC- is a mediator of microglial function and its deletion slows symptoms in amyotrophic lateral sclerosis mice. Brain. 138(Pt 1):53-68. Assay: Glutathione in mice tissue.

Yun, J et al (2015). Bergenin decreases the morphine-induced physical dependence via antioxidative activity in mice. Archives of Pharmacal Research. 38(6):1248-1254. Assay: Glutathione in mice brain and liver tissue.

Kaur C, et al (2010). Melatonin protects periventricular white matter from damage due to hypoxia. J Pineal Res. 48(3):185-93. Assay: Glutathione in rat brain tissue.

Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Glutathione in human blood.

Ogunrinu TA, Sontheimer H (2010). Hypoxia increases the dependence of glioma cells on glutathione. J Biol Chem. 285(48):37716-24. Assay: Glutathione in human glioma cells.

Park, MS et al (2010). Korean Red Ginseng Protects Oxidative Injury Caused by Lead Poisoning. JGR 34(2):132-137. Assay: Glutathione in rat blood.

Park, MS et al (2010). Korean Red Ginseng Protects Oxidative Injury Caused by Lead Poisoning. JGR 34(2):132-137. Assay: Glutathione in rat blood.

Du Y, Villeneuve NF, Wang XJ, Sun Z, Chen W, Li J, Lou H, Wong PK, Zhang DD (2008). Oridonin confers protection against arsenic-induced toxicity through activation of the Nrf2-mediated defensive response. Environ Health Perspect.116(9):1154-61. Assay: Glutathione in human cell lines.

Gumpricht E, et al (2008). Resistance of young rat hepatic mitochondria to bile acid-induced permeability transition: potential role of alpha-tocopherol. Pediatr Res. 64(5):498-504. Assay: Glutathione in rat mitochondrial GSH.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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QuantiChrom™ Glutathione (GSH) Assay Kit
Catalog No: DIGT-250
Price: $339    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4°C

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