EnzyChrom™ Glutamate Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of glutamate and evaluation of drug effects on glutamate metabolism.

Key Features
Sensitive and accurate. Detection limit of 50 μM, linearity up to 2.5 mM glutamate in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
OD565nm

Samples
Serum, plasma, urine, cell, tissue etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
50 μM

Shelf Life
6 months

More Details
Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable. BioAssay Systems’ EnzyChrom™ glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.
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1. Iscla, I et al (2014). Streptomycin potency is dependent on MscL channel expression. Nature Communications. 5:4891. Assay: Vector in E. Coli (Pubmed).

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EnzyChrom™ Glutamate Assay Kit
Catalog No: EGLT-100
Price: $319    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

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Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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