QuantiChrom™ β-Glucosidase Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism.

Key Features
High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.

Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.

Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Method
OD405nm

Samples
Biological

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
2 U/L

Shelf Life
6 months

More Details
β-GLUCOSIDASE is a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems’ QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

1. How to use of DBGD-100 assay kit for human β-glucosidase activity?



The β-glucosidase assay comes with a neutal assay buffer (60 mM KPi, pH 7.0) and is suitable for enzymes that show activity around pH 7. The product of the reaction develops an intense yellow color at high pH (9-11).

The human enzyme has a pH optimum at 5.5. It is advisable to carry out the reaction in a buffer with that pH. However, because the absorbance of the colored product at pH 5 is lower than at pH 7, you have to raise the pH before measuring the absorbance.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Mallery, SR et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: β-Glucosidase in Human saliva (Pubmed).

2. Renchinkhand, G et al (2015). Identification of ?-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Bacteria in Enterococcus faecalis Assay.

3. Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: β-Glucosidase in Human saliva (Cells in Mammals) (Pubmed).

4. Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Cells in Human Assay.

To find more recent publications, please click here.
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QuantiChrom™ β-Glucosidase Assay Kit
Catalog No: DBGD-100
Price: $359    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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