EnzyChrom™ Glucose Oxidase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.

Key Features
Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.

Method
OD570nm, FL530/585nm

Samples
Cell/tissue lysate, cell culture media etc

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
0.02 (OD), 0.002U/L (FL).

Shelf Life
6 months

More Details
Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery. BioAssay Systems glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample.
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Allen, R., Rittmann, B. E., & Curtiss, R. (2019). Axenic Biofilm Formation and Aggregation by Synechocystis sp. Strain PCC 6803 Are Induced by Changes in Nutrient Concentration and Require Cell Surface Structures. Appl. Environ. Microbiol., 85(7), e02192-18. Assay: Glucose oxidase in cyanobacteria cells.

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EnzyChrom™ Glucose Oxidase Assay Kit
Catalog No: EGOX-100
Price: $289    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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