EnzyChrom™ Glucose Assay Kit II
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.

Key Features
Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate: 0.1 to 3 mM (1.8 mg/dL to 54 mg/dL) glucose for colorimetric assays.

Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 minutes. Room temperature assay. No 37°C heater is needed.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.

Method
OD340nm

Samples
Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc

Species
All

Procedure
• 20 min

Size
100 tests

Detection Limit
0.1 mM

Shelf Life
6 months

More Details
Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism.
Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery. BioAssay Systems glucose assay kit II uses an enzyme to reduce NAD. The produced NADH, measured at 340 nm, is proportional to the glucose concentration in the sample

1. What are the differences between the three EnzyChrom Glucose assay kits?



EBGL - best at detecting low levels of glucose (as low as 5 µM)
EGL2 - best at measuring high levels of glucose using a low wavelength filter (340 nm)
EGL3 - best at measuring high levels of glucose using a high wavelength filter (565 nm)

2. Which of the three EnzyChrom Glucose assay kits can be used with urine, saliva, or serum samples?



EBGL - Not recommended for urine, saliva, or serum samples
EGL2 - Compatible with urine and serum samples, not recommended for saliva samples
EGL3 - Recommended for urine, saliva, and serum samples
*Sample blanks are to be used when testing urine samples with EGL2 or EGL3



For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
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Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Glucose Assay Kit II
Catalog No: EGL2-100
Price: $289    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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