QuantiChrom™ Glucose Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.

Key Features
Sensitive and accurate. Use as little as 5 μL samples. Linear detection range 0.7 mg/dL (39 μM) to 300 mg/dL (16.6 mM) glucose in 96-well plate.

Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath.

Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples.

Method
OD630nm (Chemical)

Samples
Biological, food and beverage

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
0.7 mg/dL (39 μM)

Shelf Life
12 months

More Details
Glucose (C6H12O6) is a ubiquitous fuel molecule in biology. It is oxidized through a series of enzyme-catalyzed reactions to form carbon dioxide and water, yielding the universal energy molecule ATP. Due to its importance in metabolism, glucose level is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and automation-ready procedures for measuring glucose concentrations find wide applications in research and drug discovery. BioAssay Systems glucose assay kit is designed to measure glucose directly in serum or plasma without any pretreatment. The improved o-toluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is directly proportional to glucose concentration in the sample.

1. What is the principle of the assay?



The QuantiChrom™ Glucose Assay is based on the formation of a blue-green complex between D-glucose and o-toluidine. The carbonyl group in the open aldehyde form of D-glucose condenses with the amino group of o-toluidine to form a stoichiometric colored imino adduct.

2. Does this assay measure fructose?



The reaction is specific to aldosugars (such as glucose) but not ketosugars (such as D-fructose). For instance in an equimolar solution of glucose and fructose, this method will measure only glucose.

3. What other sugars interfere with it?



Aldosugars would interfere in this assay, such as glyceraldehyde, ribose, arabinose, xylose, mannose, galactose. However, as glucose is the main sugar in biological samples (e.g. serum, cell culture medium), the interference is most often negligible.

4. Does phenol red in culture medium interfere with the glucose assay?



No, phenol red in the culture media does not interfere with this assay.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Kazemi, M., McBreairty, L., Chizen, D., Pierson, R., Chilibeck, P., & Zello, G. (2018). A Comparison of a Pulse-Based Diet and the Therapeutic Lifestyle Changes Diet in Combination with Exercise and Health Counselling on the Cardio-Metabolic Risk Profile in Women with Polycystic Ovary Syndrome: A Randomized Controlled Trial. Nutrients, 10(10), 1387. Assay: Glucose in human plasma.

Amrithraj, A. I., Kodali, A., Nguyen, L., Teo, A. K. K., Chang, C. W., Karnani, N. & Stunkel, W. (2017). Gestational diabetes alters functions in offspring's umbilical cord cells with implications for cardiovascular health. Endocrinology, 158(7), 2102-2112. Assay: Glucose in human cells.

Chen, X., Xu, H., Wu, N., Liu, X., Qiao, G., Su, S. & Lin, X. (2017). Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. Oncotarget 8(18): 30305-30316. Assay: Glucose in human lung tissue/plasma.

McMurphy, T. B. (2017). Environmental and gene therapy approaches to improve glycemic control and promote healthy aging (Doctoral dissertation, The Ohio State University). Assay: Glucose in mice blood.

Toorie, A. M., Cyr, N. E., Steger, J. S., Beckman, R., Farah, G., & Nillni, E. A. (2016). The nutrient and energy sensor Sirt1 regulates the hypothalamic-pituitary-adrenal (HPA) axis by altering the production of the prohormone convertase 2 (PC2) essential in the maturation of corticotropin-releasing hormone (CRH) from its prohormone in male rats. Journal of Biological Chemistry, 291(11), 5844-5859. Assay: Glucose in Sprague Dewley rats plasma.

Chiu LL, Radisic M (2010). Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues. Biomaterials 31(2):226-41. Assay: Glucose in human endothelial cells.

Hedbacker, K et al (2010). Antidiabetic effects of IGFBP2, a leptin-regulated gene. Cell Metab. 11(1):11-22. Assay: Glucose in mouse blood.

Olsen AS, et al (2010). Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound Repair Regen. 18(5):532-42. Assay: Glucose in zebrafish blood.

Vaitheesvaran B, et al (2010). MKR mice have increased dynamic glucose disposal despite metabolic inflexibility, and hepatic and peripheral insulin insensitivity. Diabetologia 53(10):2224-32. Assay: Glucose in mice serum.

Cirrik S, Oner G. (2009). The effect of heavy muscle activity on renal cytoresistance in rats. Ren Fail. 31(8):683-9. Assay: Glucose in rat urine.

Li, L et al (2009). The nuclear orphan receptor COUP-TFII plays an essential role in adipogenesis, glucose homeostasis, and energy metabolism. Cell Metab. 9(1):77-87. Assay: Glucose in mouse plasma.

Sekowska, A et al (2009). Repulsion and metabolic switches in the collective behavior of bacterial colonies. Biophys J. 97(3):688-98. Assay: Glucose in bacteria cell lysate.

Sullivan JP, et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol. 36(4):386-402. Assay: Glucose in bioreactors daily media.

Zheng Y, et al (2007). Evaluation of different biomass materials as feedstock for fermentable sugar production. Appl Biochem Biotechnol. 137-140(1-12):423-35. Assay: Glucose in wood enzyme.

Jatana M, et al (2006). Inhibition of NF-kappaB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia. J Neuroinflammation 3:12. Assay: Glucose in rat plasma.

Stites T, et al (2006). Pyrroloquinoline quinone modulates mitochondrial quantity and function in mice. J Nutr. 136(2):390-6. Assay: Glucose in mice plasma.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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QuantiChrom™ Glucose Assay Kit
Catalog No: DIGL-100
Price: $249    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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