QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism.

Key Features
Fast and sensitive. Linear detection range (20 μL sample): 0.2 to 100 U/L for 15 min reaction.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD460nm

Samples
Plasma, serum, tissue and culture media, etc

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
0.2 U/L

Shelf Life
6 months

More Details
GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems’ non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
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QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit
Catalog No: DGPDH-100
Price: $309    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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